CD3 Mouse mAb (APC) - 一次抗体, specific to CD3, Mouse IgG1

Cat. No.: Ab189667

グレード＆純度 ExactAb™, Validated, Ex:650nm, Em:660nm, 5 μL/test

Application

Flow Cytometry

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG1

Clone Number

UCHT1

Conjugation

APC

概要

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow, in vivo T cell depletion in humanized mice, ex vivo T cell inhibition for xenographs and so on.

仕様

Product Name

CD3 Mouse mAb (APC) - 一次抗体, specific to CD3, Mouse IgG1

同義語

CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a

仕様と純度

ExactAb™, Validated, Ex:650nm, Em:660nm, 5 μL/test

生化学的・生理学的メカニズム

The CD3 complex mediates signal transduction.

宿主種

マウス

特異性

CD3

免疫原

Human CD3ε

ポジティブコントロール

Flow: Human peripheral blood lymphocytes

活用

APC

励起波長(nm)

Red Laser(633nm)

発光波長(nm)

660nm

Ex/Em(nm)

Ex:650nm;Em:660nm

等級

Em:660nm, Ex:650nm, ExactAb™, Validated

アイソタイプ

Mouse IgG1

ライトチェーン・タイプ

kappa

精製方法

Protein G purified

集中

5 μL/test

Product Line

生物製品

保管と配送

保管条件

Store at 2-8°C,Protected from light,Do not freeze

入荷

Wet ice,Do not freeze

安定性と保管

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

画像

Flow Cytometry: CD3 Mouse mAb (APC) (Ab189667)

CD3 Mouse mAb (APC) (Ab189667) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (APC) (Ab189667) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG Isotype Control Antibody (APC) (Ab180309).

Flow Cytometry: CD3 Mouse mAb (APC) (Ab189667)

CD3 Mouse mAb (APC) (Ab189667) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (APC) (Ab189667) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

関連ターゲット（人間）

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

作用メカニズム

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD3 Mouse mAb (APC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.