CD8 alpha Rat mAb - 一次抗体, specific to CD8 alpha, Rat IgG2a

Cat. No.: Ab095344

GRADE & PURITY Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Depletion, Flow Cytometry, Functional Assay, IF/ICC, WB

Host species

助言

Reactive species

マウス

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

Unconjugated

概要

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on.
Application
Flow: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
in vivo CD8+ T cell depletion: Use at an assay dependent concentration.
Protein Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Specifications

Product Name

CD8 alpha Rat mAb - 一次抗体, specific to CD8 alpha, Rat IgG2a

同義語

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell

仕様と純度

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

宿主種

助言

特異性

CD8 alpha

免疫原

Mouse Spleen Cells or Thymocyte Membranes

活用

Unconjugated

等級

Azide Free, Carrier Free, PBS Only, Validated

アイソタイプ

Rat IgG2a

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

See COA

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at 2-8°C,Do not freeze

入荷

Wet ice,Do not freeze

安定性と保管

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

画像

Flow Cytometry: CD8 alpha Rat mAb (Ab095344)

CD8 alpha Rat mAb (Ab095344) - Flow Cytometry
Two-color flow cytometric analysis of CD8 alpha expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or CD8 alpha Rat mAb (Ab095344; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD8 alpha (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

Use at an assay dependent concentration.

IF/ICC

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vivo CD8+ T cell depletion

Use at an assay dependent concentration.

関連ターゲット（人間）

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)