CD8 alpha Rat mAb (PE) - 一次抗体, specific to CD8 alpha

Cat. No.: Ab190209

GRADE & PURITY Validated, ExactAb™, Ex:565nm, Em:575nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

助言

Reactive species

マウス

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

PE

概要

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on.

Specifications

Product Name

CD8 alpha Rat mAb (PE) - 一次抗体, specific to CD8 alpha

同義語

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | PE Anti-Mouse CD8a (53-6.7) | PE Anti-Mouse CD8a Antibody[53-6.7] | CD8a Monoclonal Antibody (53-6.7), PE | PE anti-mouse CD8a Antibody | T8/Leu-2 T-lymphocyte differentiation antigen antibody | T8 T cell a

仕様と純度

ExactAb™, Validated, Ex:565nm, Em:575nm, 0.5 mg/mL

生化学的・生理学的メカニズム

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

宿主種

助言

特異性

CD8 alpha

免疫原

Mouse Spleen Cells or Thymocyte Membranes

ポジティブコントロール

Flow: C57BL/6 mouse splenocytes.

活用

PE

励起波長(nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

発光波長(nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

等級

Em:575nm, Ex:565nm, ExactAb™, Validated

ライトチェーン・タイプ

kappa

精製方法

Protein G purified

集中

0.5 mg/mL

保管と配送

保管条件

Store at 2-8°C,Protected from light,Do not freeze

入荷

Wet ice,Do not freeze

安定性と保管

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

画像

Flow Cytometry: CD8 alpha Rat mAb (PE) (Ab190209)

CD8 alpha Rat mAb (PE) (Ab190209) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (PE) (Ab190209) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: CD8 alpha Rat mAb (PE) (Ab190209)

CD8 alpha Rat mAb (PE) (Ab190209) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (PE) (Ab190209) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

関連ターゲット（人間）

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)