Coelenterazine 400a - Suitable for molecular biology, ≥95%(TLC) , CAS No.70217-82-2

CAS: 70217-82-2 Cat. No.: C1375502 分子式: C₂₆H₂₁N₃O 分子量: 391.46

GRADE & PURITY Suitable for molecular biology, ≥95%(TLC)

Synonyms

8-Dibenzyl-6-phenyl-imidazo[1 | 2a]pyrazin-3-(7H)-one

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

概要

Coelenterazine 400a is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.
Note: It is not recommended to store dissolved coelenterazine 400a at -20°C or -70°C. Coelenterazine 400a is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.

Specifications

同義語

8-Dibenzyl-6-phenyl-imidazo[1 | 2a]pyrazin-3-(7H)-one

仕様と純度

Suitable for molecular biology, ≥95%(TLC)

保管条件

Protected from light,Store at -20°C,Argon charged

入荷

Ice chest + Ice pads

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。

等級

Suitable for molecular biology

純度

≥95%(TLC)

名前と識別子

• カノニカル・スマイル: C1=CC=C(C=C1)CC2=C(N3C=C(N=C(C3=N2)CC4=CC=CC=C4)C5=CC=CC=C5)O
• IUPAC Name: 2,8-dibenzyl-6-phenylimidazo[1,2-a]pyrazin-3-ol
• InChIKey: XNNYOKUWNAAJQW-UHFFFAOYSA-N
• INCHI: 1S/C26H21N3O/c30-26-23(17-20-12-6-2-7-13-20)28-25-22(16-19-10-4-1-5-11-19)27-24(18-29(25)26)21-14-8-3-9-15-21/h1-15,18,30H,16-17H2
• 異性体SMILES: C1=CC=C(C=C1)CC2=C(N3C=C(N=C(C3=N2)CC4=CC=CC=C4)C5=CC=CC=C5)O
• 分子量: 391.46
• Reaxy-Rn: 36019358
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=36019358&ln=

化学的性質と物理的性質

• 溶解度: Soluble in methanol or ethanol (NOT in DMSO).
• 屈折率: n20D1.66 (Predicted)
• 沸点（°C）: 551.39° C at 760 mmHg (Predicted)
• 融点（°C）: 94-98° C
• 分子量: 391.500 g/mol
• XLogP3: 6.200
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 5
• Exact Mass: 391.168 Da
• Monoisotopic Mass: 391.168 Da
• Topological Polar Surface Area: 50.400 Ų
• Heavy Atom Count: 30
• Formal Charge: 0
• Complexity: 524.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

参考文献

1. Liu Wang, Yangyi Liu, Jing Sun, Juanjuan Su, Jing Feng, Li Miao, Weijie Zhao, Hongjie Zhang, Kai Liu.  (2025)  In Situ Ultrasound-Triggered Bioluminescence for Combined Sono/Photodynamic Immunotherapy.  ACS Nano,      [PMID:40671351] [10.1021/acsnano.5c06999]

よくある質問

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by TLC. Lot-specific values are stated on the Certificate of Analysis.

What does Suitable for molecular biology mean?

Suitable for molecular biology indicates that the product has been processed and tested for molecular biology and proteomics. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

How should this product be stored?

Store at ?20 °C, protected from light, under argon. It is supplied under an argon blanket; reseal under inert gas after each use.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 70217-82-2, the molecular formula is C26H21N3O, and the molecular weight is 391.46 g/mol. InChIKey XNNYOKUWNAAJQW-UHFFFAOYSA-N.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.