GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥90%(SDS-PAGE)≥100 U/mg protein; Protein concentration: See COA
Accession #
A0AAQ1SWW4
Protein Tag
C-His
Expression system
E. coli
Bioactivity
≥100 U/mg protein, Unit definition: The amount of enzyme required to hydrolyze > 95% of the carbohydrates in 10 µg of human IgG within 1 hour at 37°C in a total reaction volume of 10 µl is defined as one unit.
Bioactive, ActiBioPure™, High Performance, EnzymoPure™, His Tag, ≥90%(SDS-PAGE), ≥100 U/mg protein; Protein concentration: See COA ActiBioPure™,Bioactive,High Performance,His Tag,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
概要
Endo F3 is a highly specific recombinant endoglycosidase originating from Flavobacterium meningosepticum and heterologously expressed in Escherichia coli. It catalyzes the hydrolysis of glycan chains on antibodies carrying biantennary and triantennary complex-type N-glycans.
Components List
E755024
Component
100U
1KU
Storage
E755024A
Endo-β-N-acetylglucosaminidase F3
100U
1KU
-20℃. Avoid freeze/thaw cycle.
E755024B
10×GlycoBuffer 3 (500 mM Sodium acetate pH4.5)
1000μl
1000μl
-20℃. Avoid freeze/thaw cycle.
Operating Procedures
1. Before use, take out and thaw the Endo F3 reagent, then centrifuge at 10,000 rpm for 10 seconds to collect all liquid at the bottom of the tube.
2. Combine 10 µg IgG with 1 µL of 500 mM Sodium Acetate (pH 4.5), add Endo F3, and supplement with purified water to a total reaction volume of 20 µL. Mix gently and incubate the mixture at 37°C for 1 hour.
3. Incubate at 37°C for 1 hour.
4. Analyze the digested samples using SDS-PAGE or mass spectrometry.
Remark: Incubation time and enzyme dosage shall be optimized based on actual experimental conditions.
Precautions
Wear a lab coat and disposable gloves throughout the operation for personal safety and health protection. This product is for research use only.
ActiBioPure™, Bioactive, High Performance, His Tag, EnzymoPure™
仕様と純度
Bioactive, ActiBioPure™, High Performance, EnzymoPure™, His Tag, ≥90%(SDS-PAGE), ≥100 U/mg protein; Protein concentration: See COA
生化学的・生理学的メカニズム
Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.Cleaves asparagine-linke
生物活性
≥100 U/mg protein, Unit definition: The amount of enzyme required to hydrolyze > 95% of the carbohydrates in 10 µg of human IgG within 1 hour at 37°C in a total reaction volume of 10 µl is defined as one unit.
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