Detection of activated platelets by flow cytometry
Protocols
Detection of activated platelets by flow cytometry
·Reviewed·Published ·Updated ·8 min read
Summary
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Operation method
Staining of activated platelets for double labeling detection by whole blood method
Materials and Instruments
Whole blood Citrate or hirudin CD62p-FlTC CD42b-PE PBS
Move
1. Collect blood citrate or hirudin anticoagulation.
2. 10 ul CD62p-FlTC plus 10 ul CD42b-PE, plus 5 ul whole blood (sample tube); 10 ul IgG1-FITC plus 10 ul IgG1-PE, plus 5 ul whole blood (control tube); mix gently and incubate at room temperature for 15 min.
3. Add 2~3 ml PBS (1% PFA) Terminate the reaction and analyze the platelets by SSC-LOG/CD42b-PE circling.
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Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Cite this article
Aladdin Scientific. "Detection of activated platelets by flow cytometry" Aladdin Knowledge Base, updated Dec 24, 2024. https://www.aladdinsci.com/us_en/faqs/detection-of-activated-platelets-by-flow-en.html
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