Cellular experiment

This experiment is mainly used for the suspension culture method of human peripheral blood lymphocytes and for the preparation of chromosomal specimens.

Cells of each species generally have a certain number, shape and size of chromosomes. The action of colchicine can cause dividing cells to be blocked in the mid

Mouse embryonic stem cell culture can be: (1) cell preservation; (2) used for stem cell research; (3) used for cell physiology, morphology and other research; (

The micronucleus test can be used for: chromosome damage and a rapid test method for chemical toxicants that interfere with cell mitosis.

Soft agar clone formation experiments can be used in (1) basic research on cell differentiation; (2) efficacy testing of clinical tumor therapy, etc.

Cell fusion is the process by which two or more cells merge to become a single cell. Spontaneous fusion occurs naturally in both in vivo and in vitro cultured c

This experiment was mainly used to isolate and purify boar spermatogonial cells of the Long White breeding line for molecular level studies.

Any animal or plant cell has a specific morphological structure, which can be clearly distinguished under a microscope by fixing and staining it, and then the s

This test is primarily used to screen for abnormal hemoglobin.

This experiment is mainly used to identify the lipid content of cells or to identify lipid cells.

Cultures of neural stem cells can be used (1) to enable their specific differentiation into neurons, astrocytes, and oligodendrocytes; and (2) their ability to

The advantages of cell electrofusion are (1) high fusion rate, (2) non-toxicity, (3) easy operation, and (4) high viability of the fused cells in further cultur

From the inspected feed products or raw materials, a certain number of representative parts are taken according to regulations, called samples. Samples are gene

The observation and detection of cellular autophagy processes can be applied to (1) the study of cellular defense and stress regulation mechanisms; (2) the stud

Radiation self-development technology is the use of radioactive isotopes produced by the action of rays on the photographic emulsion of silver chloride crystals

Tumor necrosis factortumor necrosis factor, TNF, is a cytokine derived from macrophages and monocytes, which has antitumor activity in vivo, not only activating

Neuralstemcells (NSCs) are a class of parent cells with dividing potential and self-renewal ability, which can give rise to all types of cells of neural tissues

Primary culture of rat brain microvascular endothelial cells can be used for: (1) research on blood-brain barrier; (2) pathophysiological and molecular biologic

Rat vascular endothelial cell culture can be used for (1) studies on blood fluidity, prevention of thrombosis, and regulation of vascular tone; (2) studies on s

Rat lung fibroblast cell culture can be (1) used for cell preservation, (2) used for molecular biology research, and (3) used for gene therapy research.

Tumor cells were stimulated with hydrogen peroxide, and apoptosis was generally seen within 5 minutes of the action of hydrogen peroxide at a final concentratio

Tumour Invasion Assay (TIA) can be applied to (1) study the effects of various cytokines on the invasion and metastasis of malignant tumor cells; (2) research o

Primary culture of mouse abdominal macrophages can be applied to (1) cell preservation; (2) molecular biology research; (3) gene therapy research.

Rabbit bladder smooth muscle cell culture can be used to (1) isolate and obtain high purity rabbit bladder smooth muscle cells, (2) perform cytological characte

Primary culture of neonatal rat cardiomyocytes can be applied to (1) cell preservation, (2) for cell morphology study, and (3) for clinical research.

Primary cultures of mouse hepatocytes can be used (1) for cell preservation, (2) for molecular biology research, and (3) for gene therapy research.

Primary culture of human synoviocytes can be applied to: (1) cell preservation; (2) for physiological and morphological studies of the corresponding cells; (3)

Observation of apoptosis by transmission electron microscopy can be used to: (1) clearly observe the changes of cell structure during different periods of apopt

Primary cultures of rat hepatic stellate cells can be (1) used for cell preservation, (2) used for molecular biology research, and (3) used for gene therapy res

Rabbit intervertebral disc cell culture can be used to (1) obtain rabbit intervertebral disc cells, (2) for research on disc-related topics, and (3) for researc

This experiment focuses on primary culture of mouse hepatocytes as the first step in establishing various cell lines.

Esophageal cancer is a malignant lesion formed by abnormal proliferation of esophageal squamous or glandular epithelium. Its development generally passes throug

Rat Mammalian Osteoblast Cell Culture Cultures are used for (1) obtaining rat mammalian osteoblasts; (2) cytological mechanism study of bone repair.

Rat aortic smooth muscle cell culture can be used to (1) obtain rat aortic smooth muscle cells, (2) for vascular disease research, and (3) for arterial disease

The experimental model of tumor cell-induced angiogenesis can be used to (1) establish an in vitro experimental model to facilitate further research, and (2) ob

Rat optic nerve oligodendrocyte culture can (1) obtain rat optic nerve oligodendrocytes; (2) be used for optic nerve injury repair studies; (3) be used for olig

Rat pancreatic islet cells can be used as (1) experimental materials for in-depth study of islet cell function in the laboratory; (2) cellular screening models

This experiment was mainly used to culture porcine thyroid cells.

Mitochondria are an important organelle within the cell and are the site of cellular respiration. The energy required for various cellular activities is mainly

Electron microscopes commonly used are transmission electron microscope (TEM) and scanning electron microscope. Compared with the light microscope electron micr

Taipan Blue is a cellular reactive dye that is commonly used to test the integrity of cell membranes. It is also used to detect the survival of cells. Live cell

In animal cells, all vesicles and vesicles surrounded by membranes except mitochondria belong to the vesicular lineage, including Golgi apparatus, lysosomes, mi

Membrane structure in which proteins and lipids have relative lateral mobility; the cell membrane is composed of a phospholipid bilayer and proteins that are em

It is mainly used to measure the level of cellular energy metabolism to indirectly reflect cell proliferation.

Fractionation (Fractionation) is a gradual sedimentation by centrifugation from low to high speed. First use low speed to make the larger particles precipitate,

Fractionation (Fractionation) is a gradual sedimentation by centrifugation from low to high speed. First use low speed to make the larger particles precipitate,

Cell growth curves can be applied to (1) determine the absolute cell growth number and (2) determine cell viability.

This experiment is mainly used for routine blood tests.

Mouse oviduct oocyte collection and structural observation can be applied to recognize the structural composition of female reproductive organs and their locati

Sister chromatids are two parallel chromatids linked by a single mitotic site, formed by replication of the same chromosome during interphase of cell division,

Microphotography is a method of taking pictures through a microscope. It is one of the indispensable means for scientific research, experience exchange and arbi

Soft agar colony culture experiments of tumor cells can be used for (1) basic research on cell differentiation; (2) efficacy testing of clinical tumor therapy,

Cellular biofilm system refers to the organelles surrounded by membranes such as the cell membrane, the nuclear membrane, and the endoplasmic reticulum, Golgi a

The process of fusing two or more cells of the same or different species into a single cell under natural conditions or by artificial means (physical, chemical

In recent years, it has been found that although the short arm of the human telomeric chromosome is the site of the 28S, 18S, and 5.8S rRNA genes, called the nu

The purpose of this experiment is to understand the general morphology and growth state of animal cells in culture, and at the same time, you can master the bas

Three factors make the COS cell expression system suitable for high-level, short-term expression of proteins: (i) the plasmid containing the SV40 replication st

The baculoviruses are a class of DNA viruses that exclusively infect arthropods in nature. The virus particles are rod-shaped, the genome is a double-stranded c

Most available baculovirus vectors are constructed on a plasmid basis and provide ampicillin resistance.

Target cells with specific antigens (e.g., normal cells, tumor cells, virus-infected cells) bind to the corresponding antibody and, with the participation of co

NK cells exist in human or animal peripheral blood, spleen, lymph nodes and bone marrow, can kill certain tumor cells, viral infection target cells, without the

Currently cultured cells can be sent to each other both domestically and internationally, for which a method of transporting the cells is required. Source of co

Lymphocyte proliferation and differentiation is an important stage in the immune response process of the body. Therefore, the detection of lymphocyte proliferat

T cells in normal human peripheral blood bind directly to sheep red blood cells (SRBC) in vitro to form rosette clusters. This is because human T cell membranes

Vascular endothelial cells are not only involved in the regulation of vascular permeability and coagulation processes, but also play an important role in many p

Cultured cells are characterized by thin layers and fresh material, which are easily penetrated during fixation, and are well fixed and suitable for electron mi

This experimental method was obtained from the official website of the Fourth Military Medical University

The tissue culture cell method facilitates direct observation of living cells and is an outstanding superiority of tissue culture techniques. It allows the expe

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

It is mainly used for the testing of research samples.

This experimental method was obtained from the official website of the Fourth Military Medical University

The cutinoglobulin A (ConA) agglutination assay can be used to: detect lectin receptors on the surface of cancer cells.

Immunocytochemistry is often applied to the identification of constructed and short-term cultured cells, the detection of cell-specific antigenic components, th

This experimental method was obtained from the official website of the Fourth Military Medical University

The cell infiltration growth assay test can be applied to (1) detect cancer cell traits and (2) study the mode of cell infiltration or penetration.

Amniotic fluid contains fetal shed epithelial cells that can be cultured in vitro to test for fetal karyotype. Source: Tissue Culture and Molecular Cytology Tec

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

Morphologic observation experiments on bone grindings can be applied to (1) examine the number of intracortical bone units, the number of old bone units (2) exa

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

Use your own blood smear and select the more homogeneous portion for observation. Since leukocytes are unevenly distributed on the blood smear, with large cells

Renal epithelial cell cultures can be used: (1) to study the Na+-dependent inorganic phosphorus transport system inhibited, for example, by parathyroid hormone;

Telomerase plays an extremely important role in the stability of chromosomes and in determining the cellular life cycle, and is mainly used in (1) the developme

Cultured epidermal keratinocytes are used as (1) models of differentiation, ideal tissue constructs for organotypic cultures, and studies of cell-cell interacti

The blood is made into a thin film smear with uniform cell distribution, stained with complex dyes, classified and counted according to the morphological charac

This experiment is from the website of Basic Medical Experimental Teaching Center of Guiyang College of Traditional Chinese Medicine.

Rapidly resuscitate cells, slowly dilute and re-inoculate at high cell density Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition

Primary cell culture can be applied to (1) molecular biology; (2) cell biology; (3) genetics; (4) immunology; (5) oncology; (6) virology and other fields.

Animal Cell Culture: A Guide to Basic Experiments Chicken embryos are easier to isolate because they are larger than contemporaneous mouse embryos. As with mous

It is mainly used to detect the killing activity of effector cells on target cells.

By determining the expression of reporter genes, such as β-gal or CAT, in transfected cells, it can be determined whether or not the guide DNA has been integra

The adenoma is usually digested with enzymes, and the cancerous tissue is minced with a scalpel blade. If well-differentiated colorectal cancer specimens are no

In situ staining for β-galactosidase can be applied (1) to determine whether the guide DNA has been integrated into the host cell and (2) to trace cell-cell in

This experiment was used for primary culture of osteoblasts.

Bone marrow cells were suspended with agar or methylcellulose, and then the cells were inoculated in Petri dishes with appropriate growth factors.

Isolation of mouse embryos can be used for: (1) Culturing mouse embryos in vitro and figuring out appropriate culture conditions. (2) Using embryos for developm

This experiment is mainly used to isolate cells.

Digest monolayers of cells or disperse the original tissue with trypsin and inoculate the cells onto a substrate coated with agar. The aggregates are transferre

Bone marrow is aspirated into the culture medium. The bone marrow is maintained as an attached multilayer of cells for at least 12 weeks and up to 30 weeks. Ste

Cells in 75 cm2 culture flasks were digested with trypsin, counted and mixed with alginate solution. Alginate cell suspension was added dropwise to calcium chlo

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

This experiment is mainly used for primary culture of adipocytes.

Add the cell suspension to a round culture flask and then place the flask on a rotating rack and turn it slowly.

Brains were removed from fetal rats at 17 d or 18 d of gestation (contact the local animal ethics committee) and the brains were made into single cell suspensio

Cells were inoculated into filter membrane Petri dishes and cultured in excess culture solution in multiwell culture plates.

Inoculation of suspension cells in cell culture flasks can be used for (1) mass culture of cells and (2) providing cells with a suitable environment for growth.

Cells and microbeads were inoculated at high concentrations and then diluted, stirred and sampled as required.

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

Epithelial cells are susceptible to fibroblast contamination, the extent of which can be determined by double immunofluorescence staining using antibodies speci

The resuscitation of frozen cells can be used for: (1) In the experimental manipulation of cells, frozen cells are resuscitated and then cultured for passaging.

This protocol is based on the method of Ehaio et al. (1987) and can be modified for the isolation and culture of other epithelial cells if tissue is available.

There are two different strategies for detecting mycoplasma: the first method, which will be described in the following scheme, utilizes the fluorescent dye Hoe

The following protocol describes the use of trypsin to treat cells for passaging or collection. Cell lines are usually maintained by passaging once a week and c

Blood count analysis can be used to (1) get basic information about the patient's blood and (2) find out if the patient has an infection, anemia, or coagulation

This protocol is a modification of the force method of Hennings et al. (1980). Gestational BALB/c mice are available from Harland Sprague Dawley, and the protoc

Different lots of serum have different abilities to support cell growth, especially clonal cell growth. Testing different batches of serum and then purchasing s

Various cellular manipulations, including passaging, freezing, and isolation of primary tissues, can lead to cell death. To determine the number of surviving ce

Cells growing in monolayers proliferate, fuse into sheets, and grow all over the surface of the cell vial. Some cells can be maintained in the plateau phase for

The generalized protocol described below applies to mouse, rat, hamster, and chicken embryos. It is best to select embryos that are approximately half the time

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

The chemical treatment method is to displace calcium and magnesium ions, which play an adhesive role between tissue cells, so that the cells can be dispersed. T

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

An immunoglobulin that can pass through the placenta and exist on the surface of platelets is called platelet-associated antibody (PA-IgG). This experiment is f

Platelet RNA levels correlate with thrombopoietic status and can distinguish thrombocytopenia due to myelosuppression or increased peripheral blood destruction.

Whole blood fluorescence monolabeling can detect platelet CD62p, CD63, CD42b, CD41, CD61 and other indicators. Source: Color Atlas of Practical Flow Cytometry,

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

Source: Human Stem Cell Culture

This experiment describes the preparation of cutin A-encapsulated culture plates for growing CEF monolayers for in vivo immunostaining. Source Compact Molecular

Source: Human Stem Cell Culture

The attenuated replication-defective poxvirus Ankara (MVA), an alternative to standard poxvirus strains, can be used as a vector for the expression of exogenous

Within this section we will present some basic techniques that are applicable to most histological studies, which can be appropriately categorized as fixation,

Although electron microscopy cannot observe living cells and tissues, the advent of electron microscopy still revolutionized the development of microscopy. Sinc

In 1949 Ling and Gerard used capillary glass tubes to make microelectrodes so that one could record the electrical activity of individual nerve cells before lab

Corneal interstitial cells, known as keratocytes, exhibit a resting phenotype characterized by a distinctive dendritic morphology and a very low to near absent

Stem and progenitor cell populations are present in a variety of adult tissues, including skin, muscle, bone marrow, and fat. A growing body of research suggest

With the advent of molecular genetics techniques, important advances have been made in somatic cell hybridization: the DNA used as a probe can be obtained from

Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press

MSCs in culture are best assessed by clone formation assays and trilineage differentiation capacity toward osteoblasts, adipocytes, and chondrocytes. However, c

Digest the monolayer culture with trypsin, or take a sample from the suspension culture; prepare a coverslip and add the cells to the small chamber of the hemoc

Hybridization of biotin-labeled or digoxigenin-labeled probes to chromosomes detects degenerate chromosomes, and individual probes can be detected by double-ant

Cells were labeled continuously for 48h and sampled at intervals for radioautography. Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition

Isotope-labeled probes were hybridized with cells fixed on microscope slides, and then the hybridization sites were visualized by radiographic autoradiography.

RTCA real-time detection of live cell activity

When fusing cells in suspension or monolayer cell systems, the treatment of cells with PEG should be short to minimize cell death. Usually, a 24 h recovery peri

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

The cut tissues were placed in trypsin at 4°C for 16-18 h. After removing the trypsin, the cells were warmed and dispersed in warm medium.

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

Cells are cultured in culture chambers with suitable optical microscope characteristics. The microscope is fitted with a camera connected to a gas recorder, an

Cells were mechanically isolated from the cell monolayer and cultured for expansion at 27°C and in suspension.

Grind whole skeletal muscle treated with collagen to disperse into single muscle fibers. Cultivate single muscle fibers on Matrigel and collect the migrating ce

Chopped tissues were placed in complete medium containing collagenase for incubation, and after the tissues were broken down, collagenase was removed by centrif

Observe the culture with the naked eye and inverted microscope, and if indicated, e.g., decreased pH, remove the old medium and add as fresh medium, then place

Single organs or tissues were cut out and placed whole in cold trypsin overnight, trypsin was removed, organs or tissues were briefly incubated, cells were disp

Alginate microbead cultures based on the gelling action of calcium chloride in chondrocyte alginate suspensions.

This protocol is really to culture the isolated and obtained pancreatic vesicle epithelial cells in collagen-coated petri dishes. In turn, two-dimensional aggre

Cells are inoculated at low density, cultured until colony formation, stained (for clone formation rate and survival assays ) or used for screening when cells a

The medium was removed, trypsin was added for brief action, incubated, cells were dispersed in medium, counted, diluted and re-inoculated.

There has been a great deal of interest in forming corneas in culture, as attempts have been made to replace the rabbit eye experiments (Draze's experiments) wi

The tissue in the medium is forcefully passed through a series of sieves of decreasing pore size until a desirable suspension of single cells or small tissue ma

The feeder layer technique depends on the prevention of fibroblast overgrowth through other contacts that inhibit cell formation in the monolayer. The feeder la

Samples were aspirated from suspension cell cultures, cells were counted, and appropriate amounts of suspension cells were inoculated into fresh medium in new c

Although cells isolated from adult livers do not express all the properties of the liver parenchyma, there is no doubt that suitable cell lines can be cultured.

6 ml of Ficoll-Hypaque solution was added to a 25 ml bind-cap centrifuge tube, 9 ml of medium containing 2 × 107 cells was added on top, centrifuged, and

Inoculation of homologous or heterologous cells-such as those from mouse embryos. Pending clonal culture of the cell under test, medium density, irradiated with

Collect medium from homologous or heterologous cell lines in late logarithmic growth, filter, and dilute with fresh medium as needed.

The agar is liquid at high temperatures, but becomes a gel at 37°C. Cells are suspended in warm agar gel medium and cultured after the agar has formed a gel, f

Aspirate colonies with a spiking gun or Pasteur pipette and transfer to culture wells in culture flasks or multiwell plates.

Colonies were digested with trypsin in porcelain cloning rings, glass rings, PTFE rings, or stainless steel rings and transferred to one well of a 24-well or 12

Place the culture bottles upside down under an X-ray machine or 60Co source and shade the desired colonies with a lead block.

Both cultured and in vitro transformed cells from tumors exhibit abnormalities in growth control, such as higher saturation densities, formation of colonies in

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

Cerebellums from 4-8 neonatal rats were cut into small cubes, placed in HBSS, and digested with trypsin for 15 min at 37°C. Cells were inoculated into petri

Since the desired organ culture technique involves placing the tissue in a position ideal for gas and nutrient exchange, most of these techniques involve placin

After trypsin digestion, epidermal slices isolated from skin slices were dispersed with EDTA and then incubated with serum-free culture medium supplemented with

Breast milk and mastopexy excised tissue are suitable sources of material for normal mammary ductal epithelium, and purer epithelial cells can be obtained from

Cells were suspended in medium containing Mexisol, and such cells were seeded in Petri dishes lined with an agar (or agarose) gel base.

Although there have been many reports of continuous cell lines for human colon cancer, there are fewer reports of normal intestinal epithelial cultures.Owens et

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

Place the culture on the microscope's stage, turn on the power, select the appropriate lens and focus on the specimen. Adjust the condenser and phase contrast r

A buffy coat or another mixed cell suspension is mixed with antibody-conjugated microbeads, diluted and placed in a magnetic separation column. Cells bound to t

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

The cells are sequentially placed in a series of concentrations of MTX for several weeks, with periodic changes to fresh medium containing the same drug concent

Cultures were fixed with methanol and stained directly with undiluted Giemsa's solution, then the stain was diluted 1:10, the cultures were washed, and observed

Homogenize cells or tissues in buffer, followed by sonication. An amount of the cell suspension is mixed with Hoechst 33258 and the fluorescence is determined.

Open the microscope and calibrate the optical path. Focus on the relevant area of the specimen. Check the connection between the camera and the computer, direct

Digest the monolayer culture with trypsin, or take a sample from the suspension culture; prepare a coverslip and add the cells to the small chamber of the hemoc

The dry powder was dissolved by constant stirring, adjusted to final volume, tested for pH and conductivity, and autoclaved in predetermined portions.

Cells blocked in mid-division were fixed, swollen in hypotonic solution, dropped on slides, stained, and observed [ Rothfels and Siminovitch, 1958; Rooney and C

Three sets of cells were cultured in multiwell plates at three different cell concentrations, and cells in one plate were counted each day before reaching plate

Cells were collected, washed with D-PBSA and reconstituted into a cell suspension of 5×107 cells/ml, cell extracts were prepared and stored at -70°C.

The colorimetric method can determine the amount of total protein at any point in time. Continuous observation over a period of time can be used to determine ne

Prepare storage concentrates and freeze for preservation. Thaw, mix, aseptically filter and store when needed.

Dissolve all the dry powder in the package with appropriate amount of ultrapure water: put the container on the magnetic stirrer and add the dry powder while st

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

The cells were fixed and primary and secondary antibodies were added sequentially and visualized by UV light.

Following passaging culture, cells assume growth forms characterized by delayed, exponential or logarithmic, and stationary (plateau) phases. The logarithmic an

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

Move the culture to another incubator, turn off the power to the empty incubator, then scrub it with detergent and ethanol and turn on the heat switch in order

Dissolve proteins, mix with dye, and read O.D. after 10 min. Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition

Cells were cultured for at least one week in the absence of antibiotics, culture supernatants were transferred to the indicated cells in early logarithmic growt

A series of cell cultures was performed at three different cell concentrations and cells were counted daily until plateau. Source: Animal Cell Culture: A Guide

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

After removal of erythrocytes by dextran-promoted sedimentation, a citrate- or heparin-anticoagulated layer of whole blood or plasma is added on top of the dens

Inoculate cells at low density, incubate until colonies form, stain and count colonies Source : Animal Cell Culture : A Guide to Basic Techniques 5th Edition

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

Blood is collected, allowed to clot, and the serum is separated. Filter the serum through filters with progressively decreasing pore sizes. Dispense vials, pack

Cultures were washed several times with high concentrations of antibiotics by rinsing monolayer cultures or by resuspending non-adherent cells by centrifugation

The tissues were minced and washed, and the tissue blocks were inoculated on the surface of culture flasks or Picot dishes with a small amount of medium contain

Viability assays are used to detect potentially damaging processes such as primary isolation, cell separation, or the percentage of living cells after freeze-th

In consultation with hospital personnel, provide labeled containers with culture media and arrange to have samples taken from the operating room or pathologist.

The tissue was minced and stirred in trypsin for several hours, and the isolated cells were collected every half hour, centrifuged, and added to serum-containin

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

The prostate was removed and the specimen processed (30 min), and the prostate tissue was digested with collagenase (1 h) to collect individual cells and small

Cells are cultured to the appropriate density, labeled with 1H -thymidine deoxyriboside for 30 min, washed and fixed, and unadulterated precursors are removed i

Living cells can take up diacetylfluorescein and hydrolyze it to fluorescein, which cannot pass through the cell membrane of living cells [Rotman and Papermaste

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

The cells were treated with different concentrations of experimental reagents for 24h. After trypsin digestion, the cells are inoculated at low cell density, in

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

Cells were cultured on a small scale and then added to a culture aspiration flask pre-moderated and pre-filled with 5% CO2. The cell suspension was slowly agita

DNA degradation fracture assay for detection of apoptosis in tumor cells can be used to (1) detect apoptosis in tumor cells and (2) detect the effect of drug tr

The detection of Caspase-3 activity in apoptosis is mainly applied to (1) enzyme viability assay (2) apoptosis index assay.

Staurosporine is a potent inhibitor of protein kinase C and most other kinases, including tyrosine protein kinases. Directly inhibits topoisomerase II activity

Prepare a cell suspension from the culture solution by injecting the suspension into each of the small chambers of the unit. Place with the long side down. Rota

The source of this experiment is the official website of the Fourth Military Medical University

The source of this experiment is the official website of the Fourth Military Medical University

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

This experimental method was obtained from the official website of the Fourth Military Medical University

Transforming growth factor (TGF)-13/Smads signaling pathway related protein expression is mainly used to: (1) study the expression of related proteins in the TG

Tec (Tyrosinekinaseexpressedinhepatocelluar carcinoma) is an important non-receptor tyrosine kinase, first cloned by Mano et al, from liver cancer tissue. The h

Lymphocyte proliferation test, usually under the stimulation of antigenic substances such as mitogens and other antigenic substances, proliferation, differentia

The mixed lymphocyte proliferation assay, also known as mixed lymphocyte culture (MLC) or as mixed lymphocyte response (MLR), is commonly used in tissue matchin

Cell staining with DAPI

Natural killer (NK) cells are involved in the first line of the body's immune defense and are mainly used in (1) anti-tumor and anti-virus (2) immunomodulation

This experiment was mainly used to determine NK cell activity.

When pure formaldehyde is dissolved in aqueous solution, it automatically polymerizes into long-chain paraformaldehyde. Its length varies and the polymerization

When T cells are stimulated by non-specific mitogens (e.g. PHA, ConA) during in vitro culture, they may be transformed into lymphoblastoid cells with increased

Check the design of the formulation: if the formulation is complete, it can be used directly after the addition of serum; if the formulation is incomplete (e.g.

This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)

Cells were labeled with 3H -TdR and DNA was extracted to determine radioactivity levels by scintigraphy Source : Animal Cell Culture : A Guide to Basic Techniqu

The dye stains the cells simultaneously, providing a better method for identifying apoptotic and necrotic cells

Three-dimensional (3D) cell culture refers to the co-culture of carriers with different materials of three-dimensional structure and different kinds of cells in

Embryonic stem cells are cells with a self-renewal and totipotent differentiation ability, capable of developing and differentiating into all tissues and organs

The vesicular lineage live staining assay in cells takes advantage of the property that neutral red (neutrol red) is a specific live stain for the vesicular lin

Cell resuscitation is the process of thawing cells frozen in liquid nitrogen and then re-culturing them to restore cell growth.

The cell membrane is the barrier for the exchange of substances between the cell and the external environment, and is a semi-permeable membrane that can selecti

Telomerase is a reverse transcriptase capable of synthesizing telomeric DNA using its own RNA as a template. It is generally accepted that telomerase consists o

In higher animals, there exists a phagocytic cell line with defense function, which is composed of white blood cells such as monocytes and granulocytes, and is

Due to the high activity of maturation promoting factor (MPF), the fusion of M-phase cells with interphase cells can cause the interphase cells to show morpholo

Cells carry out many kinds of life activities in the whole life process, which are realized by various inherent structures and their components in the cells, an

The network of fibers crisscrossing the cytoplasm of eukaryotic cells is called the cytoskeleton. According to the fiber diameter, composition and assembly stru

Nuclear transfer, also known as microinjection, is a core component of cloning in mammalian technology and has been highly regarded by the scientific community

Cilia and flagellum are specialized structures extending from the surface of cells of unicellular or multicellular organisms. Inside is a shaft composed of micr

Currently, there is one main method used for the lipid display assay in cells: the Sudan Black B method for the display of lipids in cells.

Biofilm is an important structure of the cell, in eukaryotic cells, it includes the plasma membrane wrapped around the cell and the inner membrane wrapped aroun

Embryonic stem cells are cells with a self-renewal and totipotent differentiation ability to develop and differentiate all tissues and organs of the adult body,

Microtubules are a common structure in eukaryotic cells. They are hollow tubular fibers that are polymerized from heterodimers of a and beta microtubule protein

Sister chromatid exchange (SCE) is a special type of homologous recombination between two chromatids formed during the replication of the same chromosome.The me

Different amino acids have side-chain groups with different chemical properties, some with basic side-chains, some with acidic side-chains, which makes differen

Chromosomes are visible structures that appear during mitosis in eukaryotic cells, and only by obtaining chromosome specimens can karyotyping be performed to de

Western blotting, i.e. protein immunoblotting.

Polysaccharides, mucopolysaccharides and mucins in animal tissues are generally shown by PAS.

Sex chromatin preparation test can take oral mucous membrane cells, shed cells in urine, amniotic fluid cells, chorionic villus cells, etc. as the material for

In situ hybridization (ISH) refers to the use of specifically labeled nucleic acid probes with known base sequences to hybridize with homologous DNA sequences i

Apoptosis is a form of cell death distinguished from necrosis, and cells in this state have unique properties in morphology, biochemistry and molecular biology.

Acid phosphatase is found primarily in macrophages, localized in lysosomes.

The in-induced pluripotent stem cells (iPS) technology not only solves the ethical problems associated with the source of embryonic stem cells, but also avoids

Peroxidase is an enzyme abundantly present in liver, kidney, neutrophils and epithelial cells of small intestinal mucosa, more present in the peroxisomes of cel

Cryopreservation experiments of cells refer to the protection of cells cultured in vitro by adding liquid nitrogen. In order to maintain their various biologica

Cell counting is a basic technique in cell biology experiments, which is widely used to determine the growth status of cultured cells and the inoculation densit

Cell recovery assay is a process in which frozen cells are removed from - 196 °C liquid nitrogen and thawed to restore their viability.

The Morphological Observation of Cultured Cells experiment is an experiment in which the morphology of cells is observed through a microscope to make a determin

Cell growth curves are an important means of understanding the detailed process of proliferation of cultured cells and the basic laws of cell growth.

Epidermal cell culture experiments refer to the culture of epidermal cells under in vitro conditions.

Skeletal muscle cell culture experiments are those in which skeletal muscle cells are cultured under in vitro conditions.

Cell freezing refers to the use of a protective agent added to the cryopreservation solution to freeze the cells in -175 ℃ liquid nitrogen. At present, the com

The cytokinesis index is the proportion of dividing cells to all cells in a culture.

Bone-exempt muscle-derived myogenic myoblasts are capable of culturing myoblasts isolated from the skeletal muscle of several adult animals, and can be used for

Cell micrometry experiments are experiments in which the size of cells is measured, and the method of this experiment is mainly to measure the size of cells wit

The radioisotope itself can record the site and intensity of its presence in close contact with photographic latex, showing accurately the localized relationshi

Primary cultures of cortical astrocytes can be applied to (1) cell preservation and (2) functional studies of cortical astrocytes.

Calcium ions play an important role in many physiological activities. Cytofluorescence calcium ion imaging is an imaging technique that utilizes calcium ion ind

Primary culture experiments with nerve cells can be used to: acquire myelin sheaths and become nerve fibers after they have traveled some distance from the cell

Flow cytometry (FCM) can be used for: (1) basic research and clinical application of immunology, especially in combination with monoclonal antibody technology;

Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press

Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press

Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press

Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press

The Golgi method is capable of detecting minute morphological changes in the dendrites and dendritic spines of nerve cells in the brain of animals caused by dru

Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press

Primary culture experiments of dorsal root ganglia can be used for (1) the induced growth and development of periapical ganglia and the formation of extensive n

Invasion of a malignant tumor into adjacent tissues is called invasion. Detecting the ability of a tumor to invade is a way to determine the ability of a tumor

Changes in endothelial barrier function regulate vascular leakage into tissues to maintain homeostasis. Altered endothelial cell permeability is important in th

None

Cell spreading technique is a common and necessary experimental operation for cells, essential in cell counting, culture, immunofluorescence and other experimen

The DoubletFinder method identifies doublets based on the expression matrix, mixes the simulated doublets with the original cell matrix, performs dimensionality

The human endometrium is a highly regenerative tissue, and this great regenerative capacity is thought to be directly related to human endometrial stem cells. E

Fluorescence Resonance Energy Transfer (FRET)

Cell freezing and resuscitation can be (1) used for biological preservation, (2) used for stem cell research in medicine, and (3) used for passaging culture.

Single-cell spreading plate is to add appropriate amount of complete medium into each well of cell culture plate in advance, shake well to make the medium infil

Cellular energy metabolism, specifically cellular aerobic respiration and glycolysis, was measured using the seahorse instrument.

Mouse peritoneal macrophage cultivation can (1) phagocytosis and removal of foreign substances; (2) secretion of bioactive substances; (3) to bionic fully degra

The MTT assay is a common method for cell growth detection by MTT (chemical name 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide, trade name thiazolyl

Copper-dependent cell death is a new form of cell death, defined as a non-apoptotic cell death pathway. In healthy cells, copper ions enter the cell through cop