MANF Mouse mAb: FAQs on Applications & Protocols

MANF Mouse mAb is a monoclonal antibody raised in mouse against MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor), designed to support reliable detection of MANF protein across a range of standard immunological applications.

Product Name

MANF Mouse mAb

SKU

Ab349852

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor)

Reference Clone

KC-3390 (absea)

Recommended Applications

Please refer to the product datasheet for validated applications

What Is MANF and Why Is It an Important Research Target?

MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) is a secreted protein that plays a critical role in the survival and maintenance of dopaminergic neurons in the ventral midbrain. It is also recognized as a key regulator of endoplasmic reticulum (ER) stress responses, exerting cytoprotective effects in multiple cell types. Research interest in MANF spans neurodegenerative diseases such as Parkinson's disease, diabetes-related beta-cell protection, and cardiac ischemia-reperfusion injury, making it a high-value target for both basic and translational research.

Using a validated monoclonal antibody such as the MANF Mouse mAb (Ab349852) ensures specificity and reproducibility when investigating MANF expression, localization, and regulation in experimental models.

What Applications Is MANF Mouse mAb Validated For?

The MANF Mouse mAb (Ab349852) is a monoclonal antibody optimized for immunological detection of MANF protein. Common applications for anti-MANF monoclonal antibodies of this class include Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), and Enzyme-Linked Immunosorbent Assay (ELISA). However, researchers should always consult the official product datasheet supplied with Ab349852 for the full list of experimentally validated applications, recommended dilutions, and compatible sample types, as validated conditions may vary by lot.

If you require confirmation of a specific application not listed on the datasheet, please contact Aladdin Scientific technical support for guidance.

How Should MANF Mouse mAb Be Stored and Handled?

Proper storage is essential to maintain antibody activity and ensure consistent experimental results. As a general guideline for monoclonal antibodies, MANF Mouse mAb should be stored at the temperature specified on the product datasheet and Certificate of Analysis (CoA). Most liquid antibody formulations are recommended for storage at 4°C for short-term use and at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided, as they can reduce antibody efficacy; aliquoting the stock into single-use volumes upon first thaw is strongly recommended.

Always refer to the specific storage conditions stated on the Ab349852 product page or CoA, as formulation details (e.g., glycerol content, carrier protein, preservatives) may influence optimal handling procedures.

General Protocol Guidance for MANF Mouse mAb in Western Blot

The following represents general best-practice guidance for using a mouse monoclonal antibody in Western Blot. Researchers should validate and optimize conditions specific to their experimental system:

• Sample preparation: Lyse cells or tissue in an appropriate lysis buffer containing protease inhibitors. Determine protein concentration and load 20–50 µg total protein per lane.
• Electrophoresis: Separate proteins on SDS-PAGE using a gel percentage appropriate for the expected molecular weight of MANF (approximately 20 kDa; verify on datasheet).
• Transfer: Transfer proteins to PVDF or nitrocellulose membrane using wet or semi-dry transfer.
• Blocking: Block membrane for 1 hour at room temperature with 5% non-fat milk or BSA in TBST.
• Primary antibody incubation: Incubate with MANF Mouse mAb at the dilution recommended on the Ab349852 datasheet, typically overnight at 4°C.
• Secondary antibody: Use an anti-mouse HRP-conjugated secondary antibody at the manufacturer's recommended dilution.
• Detection: Visualize using an ECL substrate appropriate for your imaging system.

Always optimize dilutions and incubation conditions empirically for your specific sample type and detection system.

Ordering, Shipping, and Returns for MANF Mouse mAb

MANF Mouse mAb is available from Aladdin Scientific under catalog number Ab349852. To place an order, visit the product page on the Aladdin Scientific website or contact your regional sales representative. Bulk and custom quantities may be available upon request.

Shipping: Antibodies are typically shipped with appropriate cold-chain packaging (wet ice or dry ice) to maintain product integrity during transit. Estimated delivery times depend on your location and applicable regional logistics; please refer to Aladdin Scientific's shipping policy or contact customer service for details specific to your region.

Returns and refunds: If you experience quality concerns with Ab349852, please contact Aladdin Scientific customer service within the timeframe specified in our returns policy, and provide your order number, a description of the issue, and supporting experimental data where possible. Our technical team will review your case promptly.

Frequently asked questions

What is the target of MANF Mouse mAb (Ab349852)?

MANF Mouse mAb targets MANF, also known as Mesencephalic Astrocyte-derived Neurotrophic Factor, a secreted protein involved in dopaminergic neuron survival and endoplasmic reticulum stress regulation. It is also referred to as ARMET (Arginine-rich, Mutated in Early Stage Tumors) in some literature.

Which species reactivity does MANF Mouse mAb cover?

Species reactivity for Ab349852 should be confirmed by consulting the official product datasheet, as validated reactivity data are lot-specific and determined experimentally. Common reactivity profiles for anti-MANF antibodies include human and mouse, but please verify this information directly on the Ab349852 product page or CoA before use.

How should I optimize the dilution of MANF Mouse mAb for my experiment?

Start with the dilution range recommended on the Ab349852 datasheet for your intended application. Perform a titration experiment using serial dilutions to identify the optimal signal-to-noise ratio for your specific sample type, fixation method, and detection system. Aladdin Scientific technical support is available to assist with troubleshooting if standard recommended conditions do not yield satisfactory results.

Can MANF Mouse mAb be used for immunoprecipitation (IP)?

Suitability of Ab349852 for immunoprecipitation should be confirmed on the official product datasheet. Not all monoclonal antibodies are validated for IP, as this application requires the antibody to recognize native, non-denatured protein epitopes. If IP is not listed as a validated application, contact Aladdin Scientific technical support before attempting this application.

What controls should I use when working with MANF Mouse mAb?

When using Ab349852, include a positive control (a cell line or tissue known to express MANF), a negative control (MANF-knockout cells or a sample known to lack MANF expression), and an isotype control (a mouse IgG of the same subclass at the same concentration) to confirm signal specificity and rule out non-specific binding. These controls are essential for data interpretation and publication.

References

1. Lindholm P, Saarma M. Novel CDNF/MANF family of neurotrophic factors. Dev Neurobiol. 2010;70(5):360-371. doi:10.1002/dneu.20760
2. Glembotski CC, Thuerauf DJ, Huang C, Vekich JA, Gottlieb RA, Doroudgar S. Mesencephalic astrocyte-derived neurotrophic factor protects the heart from ischemic damage and is selectively secreted upon sarco/endoplasmic reticulum calcium depletion. J Biol Chem. 2012;287(31):25893-25904. doi:10.1074/jbc.M112.356345
3. Parkash V, Lindholm P, Peränen J, et al. The structure of the conserved neurotrophic factors MANF and CDNF explains why they are bifunctional. Protein Eng Des Sel. 2009;22(4):233-241. doi:10.1093/protein/gzn080

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.