PDLIM5 Mouse mAb: Applications, Specificity & Protocol Guide

PDLIM5 Mouse mAb is a highly specific monoclonal antibody targeting the PDZ and LIM domain-containing protein 5 (PDLIM5), enabling reliable detection and functional studies in a range of immunological and biochemical assays.

Product Name

PDLIM5 Mouse mAb

SKU

Ab349189

Host Species

Mouse

Antibody Format

Monoclonal antibody (mAb)

Target Protein

PDLIM5 (PDZ and LIM domain protein 5)

Primary Reference Number

absea#KC-1903

Target Biology: What Is PDLIM5?

PDLIM5 (PDZ and LIM domain protein 5), also known as ENH (Enigma Homologue), is a scaffolding adaptor protein that belongs to the PDZ-LIM family. It contains an N-terminal PDZ domain and multiple C-terminal LIM domains, structural motifs that mediate protein–protein interactions critical for cytoskeletal organization and intracellular signal transduction.

PDLIM5 is expressed in a variety of tissues including heart, skeletal muscle, brain, and kidney, where it plays roles in the assembly and maintenance of the actin cytoskeleton. Through its LIM domains, PDLIM5 interacts with protein kinase C (PKC) isoforms and other signaling molecules, positioning it as an important regulator of cardiac hypertrophy and stress response pathways.

Dysregulation of PDLIM5 has been implicated in cardiovascular disease, psychiatric conditions, and cancer, making it an increasingly important target for translational research.

Antibody Characteristics & Validation

Ab349189 is a mouse monoclonal antibody (mAb) raised against PDLIM5. Monoclonal antibodies offer exceptional lot-to-lot consistency and epitope specificity compared with polyclonal preparations, making them the preferred reagent format for quantitative and reproducible experiments.

The monoclonal format ensures that every molecule in the preparation recognizes the same epitope on PDLIM5, minimizing background signal and cross-reactivity with closely related PDZ-LIM family members such as PDLIM1, PDLIM2, or PDLIM7. Researchers relying on Ab349189 can expect consistent performance across experimental replicates and between laboratory sites.

This antibody is referenced under the supplier code absea#KC-1903, which can be used for cross-referencing with distributor and manufacturer databases. For the most current information on validated species reactivity, isotype, and recommended dilutions, consult the official Aladdin Scientific product datasheet for Ab349189.

Recommended Applications & Protocol Guidelines

PDLIM5 Mouse mAb (Ab349189) is suitable for standard immunological detection methods used in mammalian cell and tissue research. Typical applications for anti-PDLIM5 monoclonal antibodies of this class include:

• Western Blot (WB): Denature and resolve cell or tissue lysates by SDS-PAGE, transfer to PVDF or nitrocellulose membrane, and probe with Ab349189. A band corresponding to the predicted molecular weight of PDLIM5 (approximately 50–65 kDa depending on isoform) is expected. Always include a positive control lysate known to express PDLIM5, such as heart or brain tissue.
• Immunohistochemistry (IHC): Use on formalin-fixed, paraffin-embedded (FFPE) or frozen sections. Antigen retrieval (heat-induced, citrate buffer pH 6.0 is commonly employed) may be required for FFPE samples. Titrate antibody concentration empirically for optimal signal-to-noise ratio.
• Immunoprecipitation (IP): Add Ab349189 to pre-cleared cell lysate, incubate with Protein G or Protein A agarose beads, and elute the immunocomplex for downstream analysis by WB or mass spectrometry.

Always refer to the current product datasheet for application-specific validated dilutions and recommended buffers, as these may be updated following new validation data.

Storage, Handling & Quality Considerations

Proper storage and handling of monoclonal antibodies are essential to preserve reagent activity and ensure data reproducibility. For Ab349189, adhere to the following general best practices unless the product datasheet specifies otherwise:

• Storage temperature: Store at –20 °C for long-term preservation. Avoid repeated freeze–thaw cycles by preparing single-use aliquots upon first thaw.
• Buffer compatibility: Antibodies are typically supplied in PBS or TBS with a carrier protein (e.g., BSA or glycerol) to stabilize the immunoglobulin. Do not dilute the antibody stock in water alone.
• Working dilution preparation: Prepare fresh dilutions in the recommended assay buffer on the day of use. Discard unused diluted antibody.
• Quality control: Aladdin Scientific performs QC testing on each antibody lot. Lot-specific data (e.g., SDS-PAGE purity, functional ELISA titers) are available on request or via the online product page.

If unexpected results are observed, consult the Aladdin Scientific technical support team, referencing the product SKU Ab349189 and the lot number printed on the vial label.

Frequently asked questions

What species reactivity does PDLIM5 Mouse mAb (Ab349189) cover?

The validated species reactivity for Ab349189 should be confirmed in the current product datasheet available on the Aladdin Scientific website. Mouse monoclonal antibodies against human PDLIM5 commonly show cross-reactivity with mouse and rat orthologs due to high sequence homology, but this must be confirmed experimentally for each lot.

Which positive control samples are recommended for Western blot with Ab349189?

Tissue or cell lysates with documented high PDLIM5 expression are ideal positive controls. Based on published expression data, cardiac tissue, skeletal muscle, and brain lysates are commonly used. Verify expression levels in your specific cell or tissue model before selecting a positive control.

How should I troubleshoot high background signal in IHC with PDLIM5 Mouse mAb?

High background in IHC is commonly caused by insufficient blocking, excessive primary antibody concentration, or inadequate washing. Increase blocking time (1–2 hours with 5–10% normal serum or BSA), optimize antibody dilution, and ensure thorough wash steps between incubations. For mouse primary antibodies on mouse tissue, use a mouse-on-mouse (M.O.M.) blocking kit to reduce endogenous immunoglobulin background.

Can Ab349189 be used for immunofluorescence (IF) or flow cytometry (FC)?

Suitability for immunofluorescence or flow cytometry has not been confirmed from the available source material for Ab349189. Please consult the official product datasheet or contact Aladdin Scientific technical support to verify whether these applications have been validated for this specific antibody lot.

What is the difference between using a monoclonal versus a polyclonal antibody for PDLIM5 detection?

Monoclonal antibodies like Ab349189 recognize a single epitope, providing high specificity and excellent lot-to-lot reproducibility, which is especially important in quantitative assays. Polyclonal antibodies recognize multiple epitopes and may offer stronger signal in some applications but can exhibit greater variability between lots and higher risk of cross-reactivity with related proteins such as other PDZ-LIM family members.

References

1. Aladdin Scientific product page: PDLIM5 Mouse mAb, SKU Ab349189 (absea#KC-1903).
2. Nakagawa N et al. The LIM domain of enigma is involved in cardiac hypertrophy. J Mol Cell Cardiol. — General reference for PDLIM5/ENH biology; specific volume, pages and year require verification.
3. Krcmery J et al. Nucleocytoplasmic functions of the PDZ-LIM protein family: new insights into organ development. Bioessays. — General PDZ-LIM family reference; specific details require verification.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.