POLR2J Mouse mAb: Key Facts, Protocol & FAQs

POLR2J Mouse mAb is a mouse monoclonal antibody targeting the RPB11 subunit (POLR2J) of human RNA polymerase II, supplied in PBS formulation and validated for immunological detection of this essential transcriptional component.

SKU

Ab350642

Clone type

Mouse monoclonal antibody (mAb)

Target protein

POLR2J (RPB11), a subunit of RNA polymerase II

Formulation

PBS (phosphate-buffered saline), no carrier protein or preservative indicated

Reference clone ID

KC-46727 (absea)

Host species

Mouse

What Is POLR2J and Why Does It Matter?

POLR2J encodes RPB11, one of the small subunits of RNA polymerase II (Pol II), the multi-subunit enzyme responsible for synthesising all messenger RNA and most non-coding RNA in eukaryotic cells. RPB11 forms a heterodimer with the RPB3 subunit, contributing to the structural assembly of the Pol II core complex. Disruption of subunit stoichiometry can impair global transcription, making POLR2J a relevant target in studies of gene regulation, transcription machinery, and diseases linked to transcriptional dysfunction.

Because POLR2J is a nuclear, constitutively expressed protein in dividing cells, antibodies against it are widely used as positive controls for nuclear fractionation experiments and as tools to study Pol II complex assembly.

Product Specifications

Catalogue number: Ab350642
Product name: POLR2J Mouse mAb
Clone reference: KC-46727 (absea)
Host: Mouse
Antibody class: Monoclonal
Formulation: PBS only — the antibody is supplied in phosphate-buffered saline without additional stabilisers, carrier proteins or sodium azide preservative as stated in the source data. Researchers should verify the exact concentration, isotype, and lot-specific quality-control data on the product datasheet or by contacting Aladdin Scientific technical support.

Note: Specific details such as antibody concentration, isotype, immunogen sequence, validated applications, and species reactivity are not fully described in the current source material. Please consult the official product datasheet (Ab350642) for complete specifications before experimental use.

Recommended Storage and Handling

Monoclonal antibodies formulated in PBS without preservatives or carrier proteins require careful handling to maintain activity. The following are general best-practice guidelines applicable to PBS-only antibody preparations; confirm lot-specific recommendations on the product datasheet:

• Short-term storage (up to 1 month): Store at 4 °C.
• Long-term storage: Aliquot and store at −20 °C or −80 °C. Avoid repeated freeze-thaw cycles, which can denature the antibody and reduce specific binding.
• Stabilisation: For long-term storage without a carrier protein, consider adding BSA (to 0.1–1%) or glycerol (to 50%) as a cryoprotectant, unless the experiment is incompatible with these additives.
• Contamination: Because no preservative is indicated, use aseptic technique and consider adding sodium azide (0.02%) if the antibody will not be used for cell-based assays.
• Avoid: Prolonged exposure to light, repeated freeze-thaw, and storage in frost-free freezers with temperature cycling.

Typical Applications

Monoclonal antibodies against POLR2J subunits are commonly employed in the following research techniques. Note that application-specific validation status for Ab350642 should be confirmed on the official datasheet:

• Western blot (WB): Detection of POLR2J protein at its expected molecular weight in cell or tissue lysates. POLR2J (RPB11) has a molecular weight of approximately 13–14 kDa.
• Immunoprecipitation (IP) / Co-IP: Isolation of Pol II complexes to study protein–protein interactions within the transcription machinery.
• Immunofluorescence (IF): Visualisation of nuclear Pol II localisation in fixed cells.
• Chromatin Immunoprecipitation (ChIP): Mapping Pol II occupancy at gene promoters and coding regions genome-wide.
• ELISA: Quantitative detection in capture-based formats.

Specific validated applications and recommended dilutions for Ab350642 must be confirmed in the product datasheet.

Ordering and Technical Support

POLR2J Mouse mAb is available from Aladdin Scientific under catalogue number Ab350642. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding validated applications, species reactivity, recommended dilutions, or lot-specific certificates of analysis (CoA), please contact Aladdin Scientific Technical Support and reference SKU Ab350642 and clone KC-46727.

Custom services such as bulk orders, conjugation, or azide-free / carrier-free reformulations may be available upon request.

Frequently asked questions

What is POLR2J and what does this antibody target?

POLR2J encodes the RPB11 subunit of RNA polymerase II, the eukaryotic enzyme that transcribes protein-coding genes into mRNA. This mouse monoclonal antibody (Ab350642) is raised against POLR2J and is designed for immunodetection of this subunit in research applications.

What formulation is POLR2J Mouse mAb (Ab350642) supplied in?

Ab350642 is supplied in PBS (phosphate-buffered saline) only, with no carrier protein or preservative indicated in the product information. Researchers handling this antibody should follow aseptic technique and consider adding a cryoprotectant for long-term storage at −20 °C or −80 °C.

Which experimental applications is this antibody suitable for?

Monoclonal antibodies targeting POLR2J are commonly used in Western blot, immunoprecipitation, immunofluorescence, ChIP, and ELISA. However, application-specific validation for Ab350642 must be confirmed by consulting the official product datasheet or contacting Aladdin Scientific Technical Support, as validated applications are not fully described in the current source material.

How should I store POLR2J Mouse mAb to preserve its activity?

For short-term use (within one month), store at 4 °C. For long-term storage, aliquot the antibody into single-use volumes and freeze at −20 °C or −80 °C. Avoid repeated freeze-thaw cycles. Since the formulation contains no preservative, use aseptic technique when handling.

What is the clone reference for Ab350642 and how can I order it?

The clone reference for POLR2J Mouse mAb is KC-46727 (absea). You can order Ab350642 directly through the Aladdin Scientific website or by contacting your regional Aladdin Scientific sales representative. For bulk orders or custom formats, please reach out to technical support.

References

1. Cramer P et al. Architecture of RNA polymerase II and implications for the transcription mechanism. Science. 2000;288(5466):640-649. doi:10.1126/science.288.5466.640
2. Aladdin Scientific product datasheet, Ab350642, POLR2J Mouse mAb, clone KC-46727.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.