Recombinant Human uPA Protein: Key FAQs & Specs

Recombinant Human uPA Protein is a full-sequence recombinant form of human urokinase-type plasminogen activator (uPA), produced for use in fibrinolysis, cell migration, and protease activity research applications.

Product Name

Recombinant Human uPA Protein

SKU

rp329645

Sequence Coverage

Full-length sequence (全序列)

Source Reference

Gxene Biotech #393

Protein Target

Urokinase-type Plasminogen Activator (uPA / PLAU)

Species

Human (Homo sapiens)

What Is Recombinant Human uPA Protein?

Urokinase-type plasminogen activator (uPA), also known as PLAU, is a serine protease that plays a central role in the fibrinolytic system. It cleaves plasminogen to generate plasmin, thereby initiating extracellular matrix degradation, cell migration, and tissue remodeling. Recombinant Human uPA Protein (SKU: rp329645) offered by Aladdin Scientific is produced using the full-length human uPA sequence, ensuring comprehensive representation of the native protein's functional domains, including the growth factor domain, kringle domain, and serine protease catalytic domain.

This recombinant protein is suitable for use in a variety of biochemical and cell-based research applications where a well-characterized, full-sequence uPA reagent is required.

Key Applications of Recombinant Human uPA Protein

Recombinant Human uPA Protein is widely used in research contexts that involve the plasminogen activation cascade and associated biological processes. Typical applications include:

• Plasminogen activation assays: Measuring the proteolytic conversion of plasminogen to plasmin under defined conditions.
• Cell migration and invasion studies: uPA-mediated extracellular matrix remodeling is critical in cancer biology and wound healing models.
• Fibrinolysis research: Investigating the dissolution of fibrin clots and thrombolytic mechanisms.
• Protein–protein interaction studies: Examining binding of uPA to its receptor uPAR (CD87) or to plasminogen activator inhibitors (PAI-1, PAI-2).
• Drug discovery and screening: As a target enzyme for serine protease inhibitor development.

Please verify suitability for specific assay formats (e.g., ELISA, SPR, activity assays) with your laboratory's validated protocols.

Storage, Handling, and Stability

Proper storage and handling of recombinant proteins are essential to maintain biological activity and prevent degradation. As general best-practice guidelines for recombinant proteins of this class:

• Upon receipt, briefly centrifuge the vial before opening to consolidate the contents.
• Reconstitute lyophilized protein according to the datasheet instructions, typically using sterile PBS or the recommended buffer.
• For short-term storage (up to 1 month), keep at 4 °C.
• For long-term storage, aliquot and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles.
• Avoid repeated freeze-thaw cycles, as these can reduce enzymatic activity and protein integrity.

Note: Specific storage conditions, reconstitution buffer, and shelf-life data for rp329645 should be confirmed on the official product datasheet available on the Aladdin Scientific website or by contacting technical support.

Ordering and Product Specifications

Recombinant Human uPA Protein is available through Aladdin Scientific under SKU rp329645. The product is based on the full-length human uPA sequence, referenced against Gxene Biotech #393.

To place an order:

• Search for SKU rp329645 on the Aladdin Scientific website.
• Select the required quantity and add to cart.
• For bulk orders, institutional pricing, or custom formulations, please contact the Aladdin Scientific sales team directly.

Important: Detailed specifications including molecular weight, expression system, purity grade, endotoxin level, and activity data are provided on the official product datasheet. Buyers are encouraged to review the datasheet and request a Certificate of Analysis (CoA) prior to purchase to confirm suitability for their specific application.

Quality, Purity, and Regulatory Considerations

Recombinant Human uPA Protein (rp329645) is intended for research use only (RUO) and is not approved for diagnostic or therapeutic use in humans. Researchers should note the following quality considerations:

• Purity: Specific purity data (e.g., SDS-PAGE or HPLC purity percentage) should be confirmed on the product datasheet or CoA.
• Endotoxin: For cell-based assays, endotoxin levels are a critical quality parameter. Please verify endotoxin specifications with Aladdin Scientific technical support.
• Expression system: The expression host (e.g., E. coli, HEK293, CHO) influences post-translational modifications and may affect activity in certain assays. Confirm the expression system on the datasheet.
• Biological activity: Activity validation data, where available, should be reviewed to ensure the protein meets your experimental requirements.

Frequently asked questions

What is the sequence coverage of Recombinant Human uPA Protein (rp329645)?

Recombinant Human uPA Protein (rp329645) is produced using the full-length human uPA sequence (全序列), as referenced against Gxene Biotech #393. This means all major functional domains of native human uPA are represented in the recombinant construct.

What are the recommended storage conditions for Recombinant Human uPA Protein?

As a general best practice for recombinant proteins, short-term storage at 4 °C (up to approximately one month) and long-term storage at −20 °C or −80 °C in aliquots are recommended to avoid repeated freeze-thaw cycles. Always refer to the official product datasheet for rp329645 for confirmed storage conditions, as these may differ from general guidelines.

What applications is Recombinant Human uPA Protein suitable for?

Recombinant Human uPA Protein is suitable for research applications including plasminogen activation assays, cell migration and invasion studies, fibrinolysis research, protein-protein interaction studies (e.g., uPA–uPAR binding), and serine protease inhibitor screening. Please verify compatibility with your specific assay format using the product datasheet and your laboratory's validated protocols.

Is Recombinant Human uPA Protein (rp329645) approved for clinical or diagnostic use?

No. Recombinant Human uPA Protein (rp329645) is designated for research use only (RUO) and is not approved for use in clinical diagnostics, therapeutic applications, or any procedure involving human subjects. It must not be used as a drug, device, or for any regulated diagnostic purpose.

How do I order Recombinant Human uPA Protein from Aladdin Scientific?

You can order Recombinant Human uPA Protein by searching for SKU rp329645 on the Aladdin Scientific website, selecting your required quantity, and proceeding through the standard checkout process. For bulk purchasing, institutional pricing, or further technical inquiries, please contact the Aladdin Scientific sales or technical support team directly.

What expression system is used to produce rp329645, and does it affect protein activity?

The specific expression system (e.g., E. coli, HEK293, or CHO cells) used to produce rp329645 is not specified in the available source material and should be confirmed on the official product datasheet or by contacting Aladdin Scientific technical support. The choice of expression host can influence post-translational modifications such as glycosylation, which may affect protein activity and suitability for certain assay formats.

References

1. Gxene Biotech Product Reference #393 — source reference for Recombinant Human uPA Protein sequence used in rp329645.
2. Dano K, et al. Plasminogen activators, tissue degradation, and cancer. Advances in Cancer Research. 1985. [General reference for uPA biology — specific citation details not confirmed from source material.]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.