FP-biotin covalently labels active serine hydrolases for ABPP studies. Learn about its mechanism, protocol tips, and applications in proteome profiling.
Topic
ABPP
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
3 resources · protocols, FAQs & articles
Step-by-step FP-biotin activity-based protein profiling protocol: probe preparation, proteome labeling, streptavidin pulldown, and SDS-PAGE/Western blot detection.
Targeted covalent inhibitors (TCIs) first form a noncovalent complex with the target protein and then form a covalent bond with a specific amino acid residue near the binding pocket. Their development focuses on increasing target occupancy and duration of action while controlling warhead ...
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Techniques often explored alongside immunological experiments.