Triphenylmethane dye-type indicators are a class of organic color-change reagents based on triarylmethane or related lactone/quinoid structures. They are widely used in acid-base titration, non-aqueous titration, complexometric titration, protein assays, histological staining, microbiological ...
Topic
Bromophenol blue
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
4 resources · protocols, FAQs & articles
In many laboratory workflows, reagents whose names include “blue” are used for tracking, staining, or colorimetric readouts. Despite similar naming, their molecular targets, signal-generation mechanisms, and susceptibility to interference differ substantially. Background elevation, unstable ...
Protein samples boiled in buffer are blue in color and turn blue-violet after being left at -80°C. What is the reason for this? Does it affect the subsequent electrophoresis?
How can I quickly remove the bromophenol blue that may be obscuring the bands at the end of the gel run?
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Techniques often explored alongside immunological experiments.