Enzyme-Coupled Reaction

Creatinine-related enzymes can convert creatinine into measurable products such as ammonia and hydrogen peroxide through direct deamination or multienzyme cascades, providing the reaction basis for biochemical renal function testing and enzyme sensor construction.

The glucose oxidase method, glucose dehydrogenase method, and hexokinase method can all be used for glucose quantification, but they differ markedly in reaction principle, readout mode, interference profile, and platform compatibility. Method selection should be based on sample type, detection ...