Tissue dissociation is the process of controllably converting solid tissues into suspensions of single cells or small cell aggregates, and it represents a key initial step for experiments such as primary cell culture, immune cell isolation, organoid construction, and single-cell omics. Compared ...
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Tissue dissociation enzyme
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
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MTT colorimetry is a detection method based on cell metabolic activity. It is widely used in cell biology and pharmacology research to evaluate cell proliferation, cell activity, cytotoxicity, and drug screening.
The DNA of apoptotic cells is cut into fragments of about 200 base pairs under the action of caspase-activated nuclease (CAD), forming DNA ladders. By detecting DNA fragmentation during apoptosis, the degree of apoptosis can be evaluated.
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Techniques often explored alongside immunological experiments.