VMAC Mouse mAb: Applications, Specificity & Protocol Guide

VMAC Mouse mAb is a mouse-derived monoclonal antibody targeting VMAC, supplied in PBS formulation and designed to deliver consistent, high-specificity performance across a range of standard immunological assays.

Product Name

VMAC Mouse mAb

SKU

Ab351446

Antibody Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Formulation

PBS (Phosphate-Buffered Saline) only

Reference Source

absea #KC-40543

What Is VMAC and Why Does It Matter?

VMAC (Vimentin-type intermediate filament-associated coiled-coil protein) is a structural protein associated with the intermediate filament cytoskeletal network. Intermediate filament proteins play pivotal roles in maintaining cellular integrity, mechanotransduction, and intracellular organization. Research into VMAC and related proteins is relevant to studies of cytoskeletal dynamics, cell differentiation, and disease states including cancer and fibrosis.

Monoclonal antibodies targeting VMAC enable researchers to detect, localize, and quantify this protein with high specificity and reproducibility, making them indispensable tools in cell biology and pathology research.

Product Specifications & Formulation

The VMAC Mouse mAb (SKU: Ab351446) is a mouse monoclonal antibody produced against the VMAC target antigen. The antibody is supplied exclusively in PBS (Phosphate-Buffered Saline) formulation without carrier proteins or preservatives unless otherwise stated, which provides versatility for downstream conjugation and labeling applications.

• Host Species: Mouse
• Antibody Format: Monoclonal (mAb)
• Formulation: PBS Only
• Reference Catalog: absea #KC-40543
• Aladdin SKU: Ab351446

Researchers should verify the specific concentration, isotype, and lot-specific quality control data on the product datasheet prior to use, as these parameters may vary by lot.

Recommended Applications & Protocol Guidelines

Mouse monoclonal antibodies such as VMAC Mouse mAb are widely employed in a variety of standard immunological techniques. While confirmed application data should be verified from the official product datasheet, the following represent common uses for antibodies of this class:

• Western Blotting (WB): Denature and separate cell or tissue lysates by SDS-PAGE, transfer to membrane, block with 5% non-fat milk or BSA in TBST, and incubate with the primary antibody at an empirically determined dilution (typically 1:500–1:2000) overnight at 4 °C. Use an appropriate anti-mouse HRP-conjugated secondary antibody for detection.
• Immunofluorescence (IF) / Immunocytochemistry (ICC): Fix cells with 4% paraformaldehyde, permeabilize with 0.1–0.3% Triton X-100, block with 5% normal serum, and incubate with the primary antibody. Detect with a fluorophore-conjugated anti-mouse secondary antibody.
• Immunohistochemistry (IHC): For formalin-fixed paraffin-embedded (FFPE) sections, perform heat-induced epitope retrieval (HIER) using citrate buffer (pH 6.0) or EDTA buffer (pH 9.0), block endogenous peroxidase activity, and apply the primary antibody followed by an appropriate detection system.
• Immunoprecipitation (IP): Pre-clear lysate, add the primary antibody, incubate with Protein A/G beads, wash, and elute for downstream analysis.

Note: Users should consult the official product datasheet for confirmed applications, validated dilutions, and positive control information specific to Ab351446.

Storage, Handling & Stability

Proper storage and handling of monoclonal antibodies are essential to preserve biological activity and ensure reproducible experimental results. The following general guidelines apply to PBS-formulated antibodies such as VMAC Mouse mAb (Ab351446):

• Short-term storage (up to 1 month): Store at 4 °C. Avoid repeated freeze-thaw cycles.
• Long-term storage: Aliquot and store at −20 °C or −80 °C. Adding a carrier protein such as 0.1% BSA may enhance stability for dilute preparations.
• Do not freeze antibody solutions that contain azide or detergent unless confirmed compatible.
• Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity.
• Centrifuge briefly before opening to collect contents at the bottom of the vial.

Always refer to the lot-specific Certificate of Analysis (CoA) for confirmed storage recommendations and expiry information.

Troubleshooting Common Issues

Even with high-quality monoclonal antibodies, experimental issues can arise. The following troubleshooting tips are applicable to VMAC Mouse mAb used in standard immunoassays:

• No signal detected: Verify antibody concentration and dilution; confirm target protein expression in your model; check secondary antibody compatibility (must be anti-mouse); ensure detection reagents are active.
• High background: Increase blocking time or concentration; optimize antibody dilution; increase wash stringency (number and duration of washes); confirm the secondary antibody does not cross-react with your sample species.
• Non-specific bands (WB): Titrate primary antibody; include appropriate negative controls; confirm correct gel percentage for target molecular weight.
• Weak or inconsistent signal (IHC/IF): Optimize antigen retrieval conditions; check fixation method; confirm permeabilization step for intracellular targets.
• Protein aggregation: Centrifuge antibody stock at 10,000 × g for 5 min before use; avoid vortexing.

Frequently asked questions

What is the formulation of VMAC Mouse mAb (Ab351446)?

VMAC Mouse mAb is supplied in PBS (Phosphate-Buffered Saline) only. This formulation is free of carrier proteins unless specified, making it suitable for a wide range of downstream applications including conjugation and labeling. Always check the lot-specific CoA for precise formulation details.

Which secondary antibody should I use with VMAC Mouse mAb?

Because VMAC Mouse mAb is produced in mouse, you should use an anti-mouse secondary antibody matched to your detection system (e.g., anti-mouse IgG HRP conjugate for Western blotting, or an anti-mouse IgG fluorophore conjugate for immunofluorescence). Ensure the secondary antibody is validated for the species of your target secondary host to avoid cross-reactivity.

How should I store VMAC Mouse mAb to maintain activity?

For short-term use (up to one month), store at 4 °C. For long-term storage, aliquot the antibody and store at −20 °C or −80 °C to prevent activity loss. Avoid repeated freeze-thaw cycles, which can cause protein aggregation and reduced performance. Refer to the product datasheet and CoA for lot-specific storage recommendations.

What applications is VMAC Mouse mAb validated for?

The specific validated applications for Ab351446 should be confirmed on the official product datasheet available from Aladdin Scientific. Mouse monoclonal antibodies of this class are commonly used in Western blotting, immunofluorescence, immunohistochemistry, and immunoprecipitation, but confirmed application data must be verified per lot.

What is the reference catalog number for VMAC Mouse mAb?

The VMAC Mouse mAb (Ab351446) is referenced as absea catalog number KC-40543. Researchers cross-referencing published literature or ordering from partner suppliers may use this reference number to identify equivalent product sources.

References

1. Product information: VMAC Mouse mAb, Ab351446, Aladdin Scientific. Referenced as absea #KC-40543, PBS formulation.
2. General principles of monoclonal antibody production and application in immunoassays — standard textbook reference (author and edition to be confirmed).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.