グレード&純度Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)See COA
Mouse anti GFP Antibody, Monoclonal antibody(F56-6A1.2.3), could be used for WB, ICC, IF and so on.
仕様
Product Name
GFP Mouse mAb - 一次抗体, specific to GFP, IgG2b
同義語
GFP antibody | Green fluorescent protein antibody | eGFP | Enhanced Green Fluorescent Protein | GFP | GFPuv | green fluorescent protein (gfp) | Green Fluorescent Protein | GFP Tag antibody | Anti- GFP Tag antibody
仕様と純度
Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA
生化学的・生理学的メカニズム
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca 2+ -activated photoprotein aequorin. Subunit struct
宿主種
マウス
特異性
GFP
免疫原
Full length enhanced GFP.
ポジティブコントロール
HEK293 cells transfected with GFP tag.
活用
Unconjugated
等級
Azide Free, Carrier Free, PBS Only, Validated
アイソタイプ
IgG2b
SDS-PAGE
26.4 kDa (Light Chain) & 50.3 kDa (Heavy Chain), under reducing conditions; 191.1 kDa, under non-reducing conditions.
精製方法
Protein G purified
集中
See COA
Product Line
生物製品
保管と配送
形状
Liquid
保管条件
Store at 2-8°C,Do not freeze
入荷
Wet ice,Do not freeze
安定性と保管
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
画像
GFP Mouse mAb (Ab105280) - Western Blot All lanes: GFP Mouse mAb (Ab105280) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2.8 s
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