PDGFC Antibody - 一次抗体, specific to PDGFC, Rabbit IgG

Cat. No.: Ab120901

グレード＆純度 Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, WB

Host species

ウサギ

Reactive species

マウス, 人間

Isotype

Rabbit IgG

Conjugation

Unconjugated

概要

Rabbit anti Human PDGFC Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50 - 1/100
Protein Function
Potent mitogen and chemoattractant for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. Appears to be involved in the three stages of wound healing: inflammation, proliferation and remodeling. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. Acts as a specific ligand for alpha platelet-derived growth factor receptor homodimer, and alpha and beta heterodimer. Binding to receptors induces their activation by tyrosine phosphorylation. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintainance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Seems to be involved in palatogenesis.

仕様

Product Name

PDGFC Antibody - 一次抗体, specific to PDGFC, Rabbit IgG

同義語

FALLOTEIN | PDGFC | PDGF-C | platelet derived growth factor C | platelet-derived growth factor C | SCDGFSpinal cord-derived growth factor | secretory growth factor-like protein | VEGF-E | Fallotein antibody | hSCDGF antibody | PDGF-C antibody | PDGFC anti

仕様と純度

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

宿主種

ウサギ

特異性

PDGFC

免疫原

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDGFC.

ポジティブコントロール

WB: A549 and HEL cell lysates. IHC: Human colon cancer tissue.

活用

Unconjugated

等級

Carrier Free, ExactAb™, Validated

アイソタイプ

Rabbit IgG

SDS-PAGE

150 kDa

精製方法

Immunogen affinity purified

集中

1.0 mg/mL

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: PDGFC Antibody (Ab120901)

PDGFC Antibody (Ab120901) - Western Blot
All lanes: PDGFC Antibody (Ab120901) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 18 kDa
Exposure time: 20.0 s

IHC: PDGFC Antibody (Ab120901)

PDGFC Antibody (Ab120901) - IHC
Immunohistochemical analysis of PDGFC staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with PDGFC Antibody (Ab120901) at room temperature and detected using an HRP conjugated compact polymer system.

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

1/500-1/1000

IHC

1/50-1/100

関連ターゲット（人間）

PDGFC Tbio Platelet-derived growth factor C (0 Activities)

Discover Target: PDGFC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PDGFC Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.