グレード&純度Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.See COA
Application
Capture Antibody, CLIA, Detection Antibody, ELISA, FIA, FLFIA, GICA, LF
Mouse anti Human PLGF Antibody, Monoclonal (7G1-2), is a Capture or Detection antibody could be used for ELISA, CLIA, LF, GICA, FIA. CLIA: Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE FLFIA: Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL
仕様
Product Name
PLGF Mouse mAb - 一次抗体, specific to PGF, Mouse IgG1
25.8 kDa (Light Chain) & 52.1 kDa (Heavy Chain), under reducing conditions; 175.6 kDa, under non-reducing conditions.
精製方法
Affinity chromatography
マッチドペア
[Ab176388] PLGF Mouse mAb as Capture or Detection antibody in ELISA, CLIA, LF, GICA, FIA
集中
See COA
Product Line
生物製品
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 37°C short term(7 days)Store at 4°C short term(30 days). Store at -20±5°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
Sandwich ELISA - PLGF protein standard curve Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176386) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176388) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence ·Capture antibody: PLGF Mouse mAb (Ab176386) ·Detection antibody: PLGF Mouse mAb (Ab176388)
Sandwich ELISA - PLGF protein standard curve Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176388) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176386) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence ·Capture antibody: PLGF Mouse mAb (Ab176388) ·Detection antibody: PLGF Mouse mAb (Ab176386)
Binding ELISA - PLGF Mouse mAb (Ab176386) Immobilized Recombinant Human PLGF Protein at 1.0 μg/mL can bind PLGF Mouse mAb (Ab176386) with the EC₅₀ of 21.40 ng/mL.
適用#テキヨウ#
適用#テキヨウ#
Dilution info
CLIA
Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
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