GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.See COA
Application
Capture Antibody, CLIA, Detection Antibody, ELISA, FIA, FLFIA, GICA, LF
Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
概要
Mouse anti Human PLGF Antibody, Monoclonal (5D9-3), is a Capture or Detection antibody could be used for ELISA, CLIA, LF, GICA, FIA. CLIA: Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE FLFIA: Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL
Specifications
Product Name
PLGF Mouse mAb - 一次抗体, specific to PGF, Mouse IgG1
25.2 kDa (Light Chain) & 51.1 kDa (Heavy Chain), under reducing conditions; 177.4 kDa, under non-reducing conditions.
精製方法
Affinity chromatography
マッチドペア
[Ab176386] PLGF Mouse mAb as Detection or Capture antibody in ELISA, CLIA, LF, GICA, FIA
集中
See COA
Product Line
生物製品
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 37°C short term（7 days）Store at 4°C short term（30 days）. Store at -20±5°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
Sandwich ELISA - PLGF protein standard curve Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176388) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176386) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence ·Capture antibody: PLGF Mouse mAb (Ab176388) ·Detection antibody: PLGF Mouse mAb (Ab176386)
Sandwich ELISA - PLGF protein standard curve Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176386) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176388) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence ·Capture antibody: PLGF Mouse mAb (Ab176386) ·Detection antibody: PLGF Mouse mAb (Ab176388)
Binding ELISA - PLGF Mouse mAb (Ab176388) Immobilized Recombinant Human PLGF Protein at 1.0 μg/mL can bind PLGF Mouse mAb (Ab176388) with the EC₅₀ of 38.54 ng/mL.
適用＃テキヨウ＃
適用＃テキヨウ＃
Dilution info
CLIA
Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
レビュー
顧客レビュー
よくある質問
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PLGF Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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