グレード＆純度Animal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is i
宿主種
マウス
特異性
Human TNF alpha
免疫原
Recombinant protein corresponding to Human TNF alpha
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
画像
Sandwich ELISA - TNF alpha standard curve Sandwich ELISA detection of Recombinant Human TNF-α Protein (rp156007) using TNF alpha Mouse mAb (Ab156547) as capture antibody at concentration of 1.0 µg/mL. Biotinylated TNF alpha Mouse mAb (Ab215301) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148). Detection range: 0.97 ng/mL - 1000.0 ng/mL
Indirect ELISA - TNF alpha standard curve Immobilized Recombinant Human TNF-α Protein (rp156007) at 1.0 µg/mL overnight at 4℃. Coated protein probed with TNF alpha Mouse mAb (Ab156547). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody. EC50: 59.41 ng/mL
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