TNF alpha Mouse mAb - 一次抗体, specific to Human TNF alpha

Cat. No.: Ab215301
注文可能
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Detection Antibody, ELISA
Host species
マウス
Reactive species
人間
Isotype
Mouse IgG1
Clone Number
C08/3E2
Conjugation
Unconjugated
★
Size
USA
ドイツ (EU)*
Price
Qty
10μg
Ab215301-10μg
4 在庫あり
—
$75.90
50μg
Ab215301-50μg
3 在庫あり
—
$223.90
100μg
Ab215301-100μg
2 在庫あり
—
$359.90
1mg
Ab215301-1mg
US製・受注生産 · 2~4週間 ·
—
$1,023.90
Enter a quantity for the sizes you want to add.
🧪
Why this grade

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Animal Free,Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

概要

Mouse anti Human TNF alpha Antibody, clone C08/3E2, could be used for a Detection Antibody in sandwich ELISAs

Specifications

Product Name
TNF alpha Mouse mAb - 一次抗体, specific to Human TNF alpha
同義語
APC1 antibody | APC1 protein antibody | Cachectin antibody | DIF antibody | Differentiation inducing factor antibody | Macrophage cytotoxic factor antibody | Tnf antibody | TNF superfamily member 2 antibody | TNF superfamily, member 2 antibody | TNF, macr
仕様と純度
Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL
生化学的・生理学的メカニズム
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is i
宿主種
マウス
特異性
Human TNF alpha
免疫原
Recombinant protein corresponding to Human TNF alpha
活用
Unconjugated
等級
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
ライトチェーン・タイプ
kappa
SDS-PAGE
150 kDa
精製方法
Protein A purified
マッチドペア
[Ab156547] TNF alpha Mouse mAb, Capture Antibody
集中
1.0 mg/mL
Product Line
biological_products
保管と配送
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
画像

Sandwich ELISA - TNF alpha standard curve
Sandwich ELISA detection of Recombinant Human TNF-α Protein (rp156007) using TNF alpha Mouse mAb (Ab156547) as capture antibody at concentration of 1.0 µg/mL. Biotinylated TNF alpha Mouse mAb (Ab215301) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.97 ng/mL - 1000.0 ng/mL

Indirect ELISA - TNF alpha standard curve
Immobilized Recombinant Human TNF-α Protein (rp156007) at 1.0 µg/mL  overnight at 4℃. Coated protein probed with TNF alpha Mouse mAb (Ab156547). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC50: 59.41 ng/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

関連ターゲット(人間)
TNF Tclin Tumor necrosis factor (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
証明書(CoA、COO、BSE/TSEと分析図)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
よくある質問と記事
ソリューション計算機
レビュー

顧客レビュー

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Animal Free grade guide → View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

関連商品

Adalimumab (anti-TNF-alpha) - 一次抗体, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to TNFSF2/TNFa, >95% (SDS-PAGE&SEC), Human IgG1, INHIBITOR of TNF-alpha inhibitor
Infliximab (anti-TNF-alpha) - 一次抗体, for ELISA,Functional Assay,Flow cytometry,Kinetics (BLI),Kinetics (SPR) various applications, specific to TNFSF2/TNFa, >95%, Human IgG1, INHIBITOR of TNF-alpha inhibitor
Goat Anti-Mouse IgG H&L Antibody - 二次抗体, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - 二次抗体, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Rabbit Anti-Mouse IgG H&L (HRP) - 二次抗体, IgG
Golimumab (anti-TNFa) - 一次抗体, specific to TNF, >95%, Human IgG1, INHIBITOR of TNF-alpha inhibitor
Rabbit Anti-Mouse IgG antibody - 二次抗体
Goat Anti-Mouse IgG H&L (FITC) - 二次抗体
Goat Anti-Mouse IgG H&L (TRITC) - 二次抗体
Goat Anti-Mouse IgG H&L Antibody - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 二次抗体
Goat Anti-Mouse IgG H&L (HRP) - 二次抗体
Goat Anti-Mouse IgG H&L (Biotin) - 二次抗体
CDP571 (anti-TNFa) - 一次抗体, specific to TNF, >95%, Human IgG4SP
Rabbit Anti-Mouse IgG (HRP) - 二次抗体
Rabbit Anti-Mouse IgG (Biotin) - 二次抗体
Goat Anti-Mouse IgG H&L (HRP) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - 二次抗体, Goat IgG
Certolizumab (anti-TNFa) - 一次抗体, specific to TNF, Human IgG1
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (AF555) - 二次抗体, specific to Mouse IgG, Goat IgG
Goat Anti-Mouse IgG H&L (AF700) - 二次抗体, specific to Mouse IgG, Goat IgG
ESBA-105 (anti-TNFa) - 一次抗体, specific to TNF, Human IgG1
hMAK195 (anti-TNF-alpha) - 一次抗体, specific to TNF, Human IgG1
TNF alpha Mouse mAb - 一次抗体, specific to Human TNF alpha
Goat Anti-Mouse IgG H&L (PE-Cy5) - 二次抗体, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - 一次抗体, specific to FXYD5
Goat Anti-Mouse IgG H&L (AF488) - 二次抗体
Goat Anti-Mouse IgG H&L (PE-Cy7) - 二次抗体
Goat Anti-Mouse IgG H&L (AF750) - 二次抗体
Goat Anti-Mouse IgG H&L (AF546) - 二次抗体
Goat Anti-Mouse IgG H&L (Oregon Green 488) - 二次抗体
Goat Anti-Mouse IgG H&L (Cy3) - 二次抗体

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.