Recombinant WTAP Antibody - Primary antibody, specific to WTAP, Rabbit IgG

Cat. No.: Ab134816
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Why this grade

ExactAb™, Validated, Recombinant, 0.6 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human WTAP Antibody, Recombinant, could be used for WB, IHC and so on.
Application
· WB: 1/500-1/1000
· IHC: 1/50-1/100
Protein Function
Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.

Specifications

Product Name
Recombinant WTAP Antibody - Primary antibody, specific to WTAP, Rabbit IgG
Synonyms
DKFZp686F20131 antibody | Female-lethal(2)D homolog antibody | FL2D_HUMAN antibody | hFL(2)D antibody | KIAA0105 antibody | MGC3925 antibody | Mum2 antibody | OTTHUMP00000017522 antibody | OTTHUMP00000017523 antibody | PNAS 132 antibody | PNAS-132 antibod
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.6 mg/mL
Host species
Rabbit
Specificity
WTAP
Immunogen
Recombinant protein of human WTAP (AA 200-396)
Positive Control
WB: HeLa, Jurkat, Hep G2 and K562 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.6 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

Recombinant WTAP Antibody (Ab134816) - Western Blot
All lanes: Recombinant WTAP Antibody (Ab134816) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 54 kDa
Exposure time: 48.8 s

Recombinant WTAP Antibody (Ab134816) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant WTAP Antibody (Ab134816). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Recombinant WTAP Antibody (Ab134816) - IF/ICC
IF analysis of Recombinant WTAP Antibody (Ab134816) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant WTAP Antibody (Ab134816) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Application
ApplicationDilution info
WB

1/500-1/1000

IHC

1/50 - 1/100

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
WTAP Tbio Pre-mRNA-splicing regulator WTAP (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505950Certificate of AnalysisMar 19, 2026 Ab134816
ZJ24F0505951Certificate of AnalysisMar 19, 2026 Ab134816
Solution Calculators
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