ExactAb™, Validated, Recombinant, 0.6 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human WTAP Antibody, Recombinant, could be used for WB, IHC and so on. Application · WB: 1/500-1/1000 · IHC: 1/50-1/100 Protein Function Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.
Specifications
Product Name
Recombinant WTAP Antibody - Primary antibody, specific to WTAP, Rabbit IgG
WB: HeLa, Jurkat, Hep G2 and K562 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.6 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant WTAP Antibody (Ab134816) - Western Blot All lanes: Recombinant WTAP Antibody (Ab134816) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa Observed band size: 54 kDa Exposure time: 48.8 s
Recombinant WTAP Antibody (Ab134816) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant WTAP Antibody (Ab134816). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant WTAP Antibody (Ab134816) - IF/ICC IF analysis of Recombinant WTAP Antibody (Ab134816) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant WTAP Antibody (Ab134816) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.