5-Carboxyfluorescein diacetate acetoxymethyl ester (5-CFDA-AM) - BioReagent,Biological Stain,for fluorescence analysis,for microscopy,≥97% , CAS No.124412-00-6

CAS: 124412-00-6 Cat. No.: C1454964 Molecular Weight: 532.45 PubChem CID: 180237

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥97%

Synonyms

5-CFDA-AM | 5-Fam(Ac)2-AM

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Staining, Fluorescence Analysis

Overview

5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell.

Specifications

Synonyms

5-CFDA-AM | 5-Fam(Ac)2-AM

Specifications & Purity

BioReagent, Biological Stain, for fluorescence analysis, for microscopy, ≥97%

Stability And Storage

Store at -20℃ long term (12 months). Store in the dark.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Biological Stain, BioReagent, for Fluorescence analysis, for Microscopy

Purity

≥97%

Names and Identifiers

• Canonical Smiles: CC(=O)OCOC(=O)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)OC(=O)C)OC5=C3C=CC(=C5)OC(=O)C)OC2=O
• IUPAC Name: acetyloxymethyl 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
• InChIKey: YDBUJZYYYBVSEF-UHFFFAOYSA-N
• INCHI: 1S/C28H20O11/c1-14(29)34-13-35-26(32)17-4-7-21-20(10-17)27(33)39-28(21)22-8-5-18(36-15(2)30)11-24(22)38-25-12-19(37-16(3)31)6-9-23(25)28/h4-12H,13H2,1-3H3
• Isomeric SMILES: CC(=O)OCOC(=O)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)OC(=O)C)OC5=C3C=CC(=C5)OC(=O)C)OC2=O
• PubChem CID: 180237
• Molecular Weight: 532.45

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Pentacarboxylic acids and derivatives

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Pentacarboxylic acids and derivatives
• Intermediate Tree Nodes: Not available
• Direct Parent: Pentacarboxylic acids and derivatives
• Alternative Parents: Xanthenes m-Phthalate esters M-phthalic acid and derivatives Diarylethers Phthalides Benzofuranones Isobenzofurans Lactones Carboxylic acid esters Oxacyclic compounds Acetals Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Pentacarboxylic acid or derivatives - Dibenzopyran - Xanthene - Meta-phthalic acid ester - Phthalate ester - Meta_phthalic_acid - Diaryl ether - Benzofuranone - Benzopyran - 1-benzopyran - Isobenzofuranone - Phthalide - Isobenzofuran - Isocoumaran - Benzenoid - Carboxylic acid ester - Lactone - Ether - Acetal - Oxacycle - Organoheterocyclic compound - Organic oxygen compound - Carbonyl group - Hydrocarbon derivative - Organic oxide - Organooxygen compound - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as pentacarboxylic acids and derivatives. These are carboxylic acids containing exactly five carboxyl groups.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Solubility in DMSO: 125 mg/mL (234.76 mM; sonication-assisted dissolution. Hygroscopic DMSO significantly affects the solubility of the product; please use freshly opened DMSO.)
• Sensitivity: Light-sensitive