GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.See COA
Sem transportador, ExactAb™, sem azida, validado, ver COA Azide Free,Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Conservar a -20°C. Evitar a congelação e descongelação repetidas Ships Arca de gelo + almofadas de gelo Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Visão geral
Anticorpo de coelho anti ARPC4 humano, policlonal, pode ser usado para WB, IHC e assim por diante. Aplicação: WB: 0,01-2µg/mL IHC: 5-20µg/mL Função da proteína Funciona como componente de ligação à actina do complexo Arp2/3 que está envolvido na regulação da polimerização da actina e, juntamente com um fator de ativação promotor da nucleação (NPF), medeia a formação de redes ramificadas de actina. Parece contactar o filamento de actina mãe
Specifications
Product Name
Anticorpo ARPC4 - Anticorpo primário, specific to ARPC4, Rabbit IgG
Sinónimos
Complexo da proteína 2/3 relacionada com a actina, subunidade 4 20 kD anticorpo | Complexo da proteína 2/3 relacionada com a actina, subunidade 4 20kDa anticorpo | Complexo da proteína 2/3 relacionada com a actina, subunidade 20-KD anticorpo | Complexo da
Especificações e pureza
Sem transportador, ExactAb™, sem azida, validado, ver COA
Espécie hospedeira
Coelho
Especificidade
ARPC4
Imunogénio
Recombinant ARPC4 expressed in E.coli (AA 3-165)
Controlo positivo
WB: HeLa, Jurkat, HEK293, THP-1, NIH/3T3 and C6 cell lysates. IHC: Human liver tissue. IF/ICC: HeLa cells.
Conjugação
Unconjugated
Grau
Azide Free, Carrier Free, ExactAb™, Validated
Isótipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificação
Antigen affinity purified + Protein A purified
Concentração
See COA
Armazenamento e envio
Forma
Líquido
Condições de armazenamento de armazenamento
Conservar a -20°C. Evitar a congelação e descongelação repetidas
Enviado em
Arca de gelo + almofadas de gelo
Estabilidade e armazenamento
Armazenar a 4 ℃ curto prazo (1-2 semanas). Armazenar a -20 ℃ a longo prazo (24 meses). Após a receção, recomenda-se a alíquota. Evite o ciclo de congelamento / descongelamento.
Imagens
ARPC4 Antibody (Ab089682) - Western Blot All lanes: ARPC4 Antibody (Ab089682) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 19 kDa Exposure time: 5.0 s
ARPC4 Antibody (Ab089682) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 20 µg/mL ARPC4 Antibody (Ab089682) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARPC4 Antibody (Ab089682) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 20 µg/mL ARPC4 Antibody (Ab089682) Control: Used PBS instead of primary antibody Second Ab: 2 µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ARPC4 Antibody (Ab089682) - IF/ICC IF analysis of ARPC4 Antibody (Ab089682) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ARPC4 Antibody (Ab089682) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Aplicação
Aplicação
Dilution info
IHC
5-20 µg/mL
WB
0.01-2 µg/mL
IF/ICC
2.3µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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