Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.
Store at -20°C,Avoid repeated freezing and thawing Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.
SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.
Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.
Mouse anti Human CASP3 Antibody, clone CD01/2F2, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.
Caspase 3 Mouse mAb (Ab156544) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab156544) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 34,36 kDa
Exposure time: 16.2 s
Caspase 3 Mouse mAb (Ab156544) - Western Blot
All lanes: Caspase 3 Mouse Antibody (Ab156544) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 34 kDa
Exposure time: 120.0 s
Caspase 3 Mouse mAb (Ab156544) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells stained with Caspase 3 Mouse mAb (Ab156544) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2a-kappa, anti-A29L (Ab210599) was used as an isotype control (Blue).
Caspase 3 Mouse mAb (Ab156544) - ICC/IF
IF analysis of Caspase 3 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Caspase 3 Mouse mAb (Ab156544) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
1/200
1/1000 - 1/200000
1/200 - 1/12500
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<<<<<<< HEAD Baixar SDS → ======= Baixar SDS → >>>>>>> release248-p3Dados de qualidade específicos do lote. Introduza o número de lote para obter o COA exato.
Consultar COA →Resumo de referência rápida das especificações e aplicações do produto.
Ver ficha técnica →Atributos de qualidade completos e critérios de aceitação para este grau.
Ver folha de especificações →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Data | Item |
|---|---|---|---|
 ZJ25F0116463 | Certificate of Analysis | Oct 09, 2026 | Ab156544 |
| ZJ25F0116464 | Certificate of Analysis | Oct 09, 2026 | Ab156544 |
| ZJ25F0116465 | Certificate of Analysis | Oct 09, 2026 | Ab156544 |
O nosso guia de seleção de graus abrange pureza, estado do estabilizador e adequação à aplicação para todas as variantes do nosso catálogo.
Ver o guia de graus Carrier Free → Ver o guia de graus Validated → Ver o guia de graus ExactAb™ → Ver o guia de graus Azide Free → Ver o guia de graus High Performance → Ver o guia de graus PBS Only →
