CD154/CD40L Mouse mAb - Anticorpo primário, specific to TRAP/CD40L, Mouse IgG2a

Cat. No.: Ab132316

GRADE & PURITY Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IP

Host species

Rato

Reactive species

Humano, Macaco

Isotype

Mouse IgG2a

Clone Number

5C8

Conjugation

Unconjugated

Visão geral

Mouse anti Human/Monkey CD154/CD40L Antibody, Monoclonal (5C8), could be used for in vitro blocking of CD40/CD40L signaling, in vivo blocking of CD40/CD40L signaling, IP, Flow and so on.

Application:
in vitro blocking of CD40/CD40L signaling: Use at an assay dependent concentration.

in vivo blocking of CD40/CD40L signaling: Use at an assay dependent concentration.

IP: Use at an assay dependent concentration.

Flow: Use at an assay dependent concentration.
Protein Function
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

Specifications

Product Name

CD154/CD40L Mouse mAb - Anticorpo primário, specific to TRAP/CD40L, Mouse IgG2a

Sinónimos

IGM | IMD3 | CD154 antigen | CD154 | CD40 antigen ligand | CD40 Ligand | CD40L | CD40-L | CD40LG | CD40LIGM | gp39 | hCD40L | HIGM1 | T-B cell-activating molecule | T-BAM | T-cell antigen Gp39 | TNF-related activation protein | TNFSF5 | TNFSF5IMD3 | TRAP

Especificações e pureza

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Espécie hospedeira

Rato

Especificidade

TRAP/CD40L

Imunogénio

Human D1.1 T cell line

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, PBS Only, Validated

Isótipo

Mouse IgG2a

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein G purified

Concentração

See COA

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at 2-8°C,Do not freeze

Enviado em

Wet ice,Do not freeze

Estabilidade e armazenamento

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Imagens

ELISA: CD154/CD40L Mouse mAb (Ab132316)

CD154/CD40L Mouse mAb (Ab132316) - ELISA
Immobilized Recombinant Human CD40 Ligand/TNFSF5 protein (rp189423) at 1.0 μg/mL can bind CD154/CD40L Mouse mAb (Ab132316) with the EC₅₀ of 3.301 ng/mL.

Aplicação

Aplicação

Dilution info

IP

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vitro blocking of CD40/CD40L signaling

Use at an assay dependent concentration

in vivo blocking of CD40/CD40L signaling

Use at an assay dependent concentration

Alvos associados (humanos)

CD40LG Tbio CD40 ligand (0 Activities)

Discover Target: CD40LG

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD154/CD40L Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.