Rat anti Mouse CD25 Antibody, Monoclonal (PC-61.5.3), could be used for Flow Cytometry
CD25 Rat mAb (AF488) (Ab211656) - Flow Cytometry
Con A-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were stained with CD25 Rat mAb (AF488) (Ab211656) (red) compared to an isotype control (blue). Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).
CD25 Rat mAb (AF488) (Ab211656) - Flow Cytometry
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were stained with CD25 Rat mAb (AF488) (Ab211656) (right panel) compared to an isotype control (left panel). Left panel - Rat IgG (AF488) (Ab190240).
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Data | Item |
|---|---|---|---|
 ZJ25F0420068 | Certificate of Analysis | Sep 30, 2026 | Ab211656 |
| ZJ25F0420069 | Certificate of Analysis | Sep 30, 2026 | Ab211656 |
| ZJ25F0420070 | Certificate of Analysis | Sep 30, 2026 | Ab211656 |
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