CD3 Mouse mAb (APC) - Anticorpo primário, specific to CD3, Mouse IgG1

Cat. No.: Ab189667

GRAU & PUREZA ExactAb™, Validated, Ex:650nm, Em:660nm, 5 μL/test

Application

Flow Cytometry

Host species

Rato

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

UCHT1

Conjugation

APC

Visão geral

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow, in vivo T cell depletion in humanized mice, ex vivo T cell inhibition for xenographs and so on.

Especificações

Product Name

CD3 Mouse mAb (APC) - Anticorpo primário, specific to CD3, Mouse IgG1

Sinónimos

CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a

Especificações e pureza

ExactAb™, Validated, Ex:650nm, Em:660nm, 5 μL/test

Mecanismos bioquímicos e fisiológicos

The CD3 complex mediates signal transduction.

Espécie hospedeira

Rato

Especificidade

CD3

Imunogénio

Human CD3ε

Controlo positivo

Flow: Human peripheral blood lymphocytes

Conjugação

APC

Excitação (nm)

Red Laser(633nm)

Emissão (nm)

660nm

Ex/Em(nm)

Ex:650nm;Em:660nm

Grau

Em:660nm, Ex:650nm, ExactAb™, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

Método de purificação

Protein G purified

Concentração

5 μL/test

Product Line

Produtos biológicos

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at 2-8°C,Protected from light,Do not freeze

Enviado em

Wet ice,Do not freeze

Estabilidade e armazenamento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imagens

Flow Cytometry: CD3 Mouse mAb (APC) (Ab189667)

CD3 Mouse mAb (APC) (Ab189667) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (APC) (Ab189667) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG Isotype Control Antibody (APC) (Ab180309).

Flow Cytometry: CD3 Mouse mAb (APC) (Ab189667)

CD3 Mouse mAb (APC) (Ab189667) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (APC) (Ab189667) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicação

Aplicação

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Alvos associados (humanos)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD3 Mouse mAb (APC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.