CD44 Rat mAb - Primary antibody, specific to CD44, Rat IgG2b

Cat. No.: Ab094854

GRADE & PURITY Carrier Free, Validated, ExactAb™, Low Endotoxin, Azide Free, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Application

CyTOF®, ELISA, Flow Cytometry, Functional Assay, IHC, IP, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

IM7

Conjugation

Unconjugated

Overview

Rat anti Mouse CD44 Antibody, Monoclonal (IM7), could be used for IP, WB, Flow Cytometry, CyTOF®, IHC, ELISA, Functional Assays and so on.  

Specifications

Product Name

CD44 Rat mAb - Primary antibody, specific to CD44, Rat IgG2b

Synonyms

Ly-24 | Pgp-1 | HERMES | CD44 antigen | CD44 molecule (Indian blood group) | CD44 | CD44R | CDw44 | cell surface glycoprotein CD44 | chondroitin sulfate proteoglycan 8 | CSPG8 | ECMR-III | epican | Extracellular matrix receptor III | GP90 lymphocyte homin

Specifications & Purity

Carrier Free, ExactAb™, Low Endotoxin, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Biochemical and Physiological Mechanisms

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA

Host species

Rat

Specificity

CD44

Immunogen

Dexamethasone-induced myeloid leukemia M1 cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, Low Endotoxin, PBS Only, Validated

Isotype

Rat IgG2b

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Flow Cytometry: CD44 Rat mAb (Ab094854)

CD44 Rat mAb (Ab094854) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD44 with CD44 Rat mAb (Ab094854) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD44 Rat mAb (Ab094854)

CD44 Rat mAb (Ab094854) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD44 (red) with CD44 Rat mAb (Ab094854) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• WB:

  Use at an assay dependent concentration.;

• Functional Assay:

  Use at an assay dependent concentration.;

• IP:

  Use at an assay dependent concentration.

• IHC:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• ELISA:

  Use at an assay dependent concentration.;

• CyTOF:

  Use at an assay dependent concentration.;

Associated Targets(Human)

CD44 Tbio CD44 antigen (0 Activities)

Discover Target: CD44

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action