CD8 alpha Rat mAb (AF488) - Anticorpo primário, specific to CD8 alpha

Cat. No.: Ab190216

GRADE & PURITY ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Conselho

Reactive species

Rato

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

AF488

Visão geral

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow Cytometry.

Specifications

Product Name

CD8 alpha Rat mAb (AF488) - Anticorpo primário, specific to CD8 alpha

Sinónimos

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell

Especificações e pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos e fisiológicos

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Espécie hospedeira

Conselho

Especificidade

CD8 alpha

Imunogénio

Mouse Spleen Cells or Thymocyte Membranes

Controlo positivo

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugação

AF488

Excitação (nm)

Blue Laser(488nm)

Emissão (nm)

525nm

Ex/Em(nm)

Ex:490nm;Em:525nm

Grau

ExactAb™, Validated

Tipo de corrente de luz

kappa

Método de purificação

Protein G purified

Concentração

0.5 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at 2-8°C,Protected from light,Do not freeze

Enviado em

Wet ice,Do not freeze

Estabilidade e armazenamento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imagens

Flow Cytometry: CD8 alpha Rat mAb (AF488) (Ab190216)

CD8 alpha Rat mAb (AF488) (Ab190216) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (AF488) (Ab190216) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF488) (Ab190240).

Flow Cytometry: CD8 alpha Rat mAb (AF488) (Ab190216)

CD8 alpha Rat mAb (AF488) (Ab190216) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (AF488) (Ab190216) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicação

Aplicação

Dilution info

Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Alvos associados (humanos)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD8 alpha Rat mAb (AF488)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.