Choleterol Esterase (CHE), CAS No.9026-00-0

CAS: 9026-00-0 Cat. No.: D776909 EC Number: 232-808-6

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥10U/mg enzyme powder; ≥200U/mg protein

Accession #

P34163,Q07950,Q07804

Bioactivity

≥10U/mg enzyme powder; ≥200U/mg protein

Overview

Source: Microorganism
Isoelectric point: 5.9 
Michaelis constant: 5.4×10^-5 M (Cholesterol ester) 
Optimum pH: 6.0                     Fig. 1
Optimum temperature: 40℃                     Fig. 3
pH Stability: 6.0~9.0 (25℃, 24hr)                     Fig. 2
Thermal stability: < 40℃ (pH 7.0, 15min)                     Fig. 4
Effect of various chemicals:                     Table 1

Reaction:

Specifications

Product Name

Choleterol Esterase (CHE), CAS No.9026-00-0

Synonyms

sterol esterase | cholesterol esterase | cholesterol ester synthase | triterpenol esterase | TGL1 | YEH2 | YEH1 | YKL5

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥10U/mg enzyme powder; ≥200U/mg protein

Bioactivity

≥10U/mg enzyme powder; ≥200U/mg protein

Accession #

P34163, Q07950, Q07804

Predicted molecular weight

52 kDa (SDS-PAGE)

CAS

9026-00-0

Enzyme Commission Number

EC 3.1.1.13

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥10U/mg enzyme powder; ≥200U/mg protein

Reconstitution

Reconstitute in water.

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ (2 years). Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

One unit will convert one micromole of cholesterol ester to cholesterol per minute at pH 7.0 at 37℃.

References

1. Tan Min, Mao Jing, Zheng Jianxiong, Meng Yu, Li Jun, Hao Jiayao, Shen Haili.  (2024)  Mammalian STE20-like kinase 1 inhibits synoviocytes activation in rheumatoid arthritis through mitochondrial dysfunction mediated by SIRT3/mTOR axis.  INFLAMMATION RESEARCH,      [PMID:38265688] [10.1007/s00011-023-01846-5]

2. Fangfang Huang, Qingfei Dai, Kewei Zheng, Qingbao Ma, Yu Liu, Shuoqi Jiang, Wei Jiang, Xiaojun Yan.  (2023)  Exploring the inhibitory potential of KPHs-AL-derived GLLF peptide on pancreatic lipase and cholesterol esterase activities.  FOOD CHEMISTRY,      [PMID:38061297] [10.1016/j.foodchem.2023.138108]

3. Hong Zhang, Wenbin Liu, Lei Wang, Xin Zhang, Bo Wang, Xu Zeng, Bin Zhang.  (2024)  Functionalized corn stalk carboxymethyl cellulose and mesoporous silica SBA-15 composite hydrogel-immobilized lipase for the resolution of racemic ibuprofen ethyl ester.  Green Chemistry Letters and Reviews,      [PMID:] [10.1080/17518253.2024.2338241]

4. Na Li, Xin Fan, Yefan Wang, Kexin Zhang, Rong Liu, Yunpeng Xu, Zhifeng Tan, Wensi Xu, Dayong Zhou, Deyang Li.  (2024)  Investigation of isomerization and oxidation of astaxanthin in ready-to-eat Litopenaeus vannamei during accelerated storage.  FOOD RESEARCH INTERNATIONAL,      [PMID:39277244] [10.1016/j.foodres.2024.114983]

5. Liuyi Wei, Taoying Wu, Hao Wu, Li Wen, Bo Cui, Yunhui Cheng.  (2026)  Unraveling novel pancreatic lipase and cholesterol esterase inhibitory peptides in Oryza Sativa L. and their mechanism by peptidomics, molecular dynamics simulations, and cell study.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2025.108227]