dITP (100mM) - BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, ≥99%, 100mM , CAS No.16595-02-1
dITP (100mM) - BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, ≥99%, 100mM , CAS No.16595-02-1
GRAU & PUREZABioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.DNase, RNase free?Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk.PCR Reagent?PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification.Suitable for molecular biology?Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents.Sterile?Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization.for DNA and RNA applications?For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk.≥99%100mM
BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, ≥99%, 100mM BioReagent,DNase, RNase free,Estéril,for DNA and RNA applications,PCR Reagent,Suitable for molecular biology para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.
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Armazenamento e envio
Store at -20°C,Avoid repeated freezing and thawing Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.
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Documentos de qualidade
SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.
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Comprovação documental
Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.
Visão geral
dITP, 2'-deoxy-inosine-5'-triphosphate, can be used in PCR, real-time PCR, RT-PCR, cDNA synthesis, as well as primer extension and SNP genotyping with Taq DNA polymerase. It is generally not used in PCR with proofreading DNA polymerase.dITP cannot replace dGTP completely in PCR mix. Otherwise, DNA amplification will be inhibited significantly. It is recommended to replace10% dGTP by dITP in PCR mix. For example, dNTP/dITP Mixture (2.5mM each + 0.25mM dITP) can be used in PCR with high fidelity DNA polymerase .The molecular formula of dITP is C10H12N4O13P3Na3 with a molecular weight of 558.11 (492.1 in acid form).This dITP solution is prepared with ultrapure water to a concentration of 100mM. The pH is titrated to 7.0-7.5 with high-purity NaOH solution.This product is free of DNase, RNase, phosphatase and protease, and can be directly used for different molecular biology applications.
Precautions:
This product can be thawed at room temperature. Keep it on ice for use and store at -20℃ immediately after use.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.
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Perguntas frequentes
What is the purity of this product?
This product is supplied at ≥99% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.
What does BioReagent mean?
BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
What does DNase, RNase free mean?
DNase, RNase free indicates that the product has been processed and tested for sensitive nucleic acid and protein workflows. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 16595-02-1, the molecular formula is C10H12N4O13P3Na3, and the molecular weight is 558.11 g/mol.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
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