GRAU & PUREZACarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.
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Armazenamento e envio
Store at -20°C,Avoid repeated freezing and thawing Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.
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Documentos de qualidade
SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.
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Comprovação documental
Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.
Visão geral
Rabbit anti Human DOK7 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.
Especificações
Product Name
DOK7 Antibody - Anticorpo primário, specific to DOK7, Rabbit IgG
Sinónimos
DOK 7 antibody | Docking protein 7 antibody | DOK7 antibody | DOK7_HUMAN antibody | Downstream of tyrosine kinase 7 antibody | Protein Dok-7 antibody | CMS10 | CMS1B | FADS3 | C4orf25
Especificações e pureza
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Espécie hospedeira
Coelho
Especificidade
DOK7
Imunogénio
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DOK7.
Controlo positivo
WB: Mouse brain, Rat brain lysates. IHC: Human brain tissue. ICC/IF: Hep G2 cells.
Conjugação
Unconjugated
Grau
Carrier Free, ExactAb™, Validated
Isótipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificação
Immunogen affinity purified
Concentração
1.0 mg/mL
Product Line
Produtos biológicos
Armazenamento e envio
Forma
Liquid
Condições de armazenamento de armazenamento
Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imagens
DOK7 Antibody (Ab100303) - Western Blot All lanes: DOK7 Antibody (Ab100303) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/10000 dilution
Predicted band size: 53 kDa Observed band size: 56 kDa Exposure time: 32.0 s
DOK7 Antibody (Ab100303) - IF/ICC Immunofluorescent analysis of DOK7 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with DOK7 Antibody (Ab100303) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DOK7 Antibody (Ab100303) - IHC Immunohistochemical analysis of DOK7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with DOK7 Antibody (Ab100303) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Aplicação
Aplicação
Dilution info
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
WB
1/500-1/1000
Documentação
📋 Ficha de dados de segurança (FDS)
Documento abrangente sobre perigos, manuseamento, armazenamento e conformidade regulamentar.
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