Laboratory Protocols & SOPs Library

Acrylamide (40%) Recipe

· Published 27/07/2023 · Updated 09/06/2025

Recipe

1.Dissolve 380g of acrylamide and 20g of N,N'-methylbisacrylamide in 600ml of H2O. Heating may be necessary to dissolve the acrylamide.

2.Adjust the volume to 1L with H2O.

3.Sterilize the solution by filtration (0.45 micron pore size).

4.Check the pH (should be 7.0 or less).

5.Store in dark bottles at room temperature.

Caution: Acrylamide is a neurotoxin. Use extreme caution when handling anything with acrylamide and bisacrylamide. Protective equipment should be worn.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Acrylamide (40%) Recipe" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/us_pt/faqs/acrylamide-40-percent-recipe-en.html

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