Laboratory Protocols & SOPs Library

B5 Fixative Recipe

· Published 27/07/2023 · Updated 09/06/2025

Caution: Mercuric chloride is a skin and eye irritant and Formaldehyde is a carcinogen. Use protective equipment and prepare under hood in a well ventilated area. Reference proper procedures and disposal.

Recipe

1.Stock solution (stable for approximately 1 year). Mix well and do not use any metal utensils or foil lined lids.

12.0g Mercuric chloride

2.5g Sodium acetate

200ml Distilled water 200 ml

2.Working solution (prepare right before use)

20ml of stock solution

2ml of formaldehyde

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "B5 Fixative Recipe" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/us_pt/faqs/b5-fixative-recipe-en.html

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