Suspend 43g of the powder in 1L of distilled water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Optional: To prepare blood agar, aseptically add 5% sterile defibrinated blood to the medium at 45-50°C. Mix well and dispense as desired.
Component
g/liter
Pancreatic Digest of Casein
5
Proteose Peptone No. 3
2
Pancreatic Digest of Gelatin
3
Beef Extract
3
Dextrose
10
Sodium Chloride
5
Agar
15
Total: 43g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
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