Laboratory Protocols & SOPs Library

Field's Stain

· Published 27/07/2023 · Updated 09/06/2025

Recipe

Field's Stain #1

1.Dissolve 1.6 g of methylene blue in 1 liter of distilled water

2.Dissolve 2.6g of Na2HPO4 (anhydrous) to the solution from step 1

3.Dissolve 1g of Azure 1 in the solution from step 2

4.Dissolve 2.6 g of KH2PO4 in the solution from step 3

5.Place on mild heat with stirring or shaking for 45 minutes to 1 hour

6.Let stand at room temperature for 24 hours

7.Filter

Field's Stain #2

1.Dissolve 2g of Eosin Y in 1 liter of distilled water

2.Dissolve 2.6g of Na2HPO4 in the solution from step 1

3.Dissolve 2.6g of KH2PO4 in the solution from step 2

4.Filter

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Aladdin Scientific. "Field's Stain" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/us_pt/faqs/fields-stain-recipe-en.html

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