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Laboratory Protocols & SOPs Library
Phosphate Buffered Saline (PBS) 1x

Laboratory Protocols & SOPs Library

Phosphate Buffered Saline (PBS) 1x

· Published 27/07/2023 · Updated 09/06/2025

Recipe

1.Dissolve the following in 800ml distilled H2O.

8g of NaCl
0.2g of KCl
1.44g of Na2HPO4
0.24g of KH2PO4

2.Adjust pH to 7.4.

3.Adjust volume to 1L with additional distilled H2O.

4.Sterilize by autoclaving

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Phosphate Buffered Saline (PBS) 1x" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/us_pt/faqs/phosphate-buffered-saline-pbs-1x-en.html

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