Recombinant Human GATM Protein: FAQs & Key Facts

Recombinant Human GATM Protein is a recombinant form of the human glycine amidinotransferase enzyme involved in the first committed step of creatine biosynthesis, widely used in metabolic and enzymatic research.

Product Name

Recombinant Human GATM Protein

SKU

rp369535

Gene Name

GATM (also known as AGAT)

Protein Function

Catalyzes the transfer of an amidino group from arginine to glycine, producing guanidinoacetate and ornithine — the first step in creatine biosynthesis

Primary Expression Sites

Kidney and liver (in vivo)

Supplier Reference

absea#PP-3876

What Is Recombinant Human GATM Protein?

Recombinant Human GATM Protein is a recombinantly produced form of the human glycine amidinotransferase (GATM), also referred to as arginine:glycine amidinotransferase (AGAT). This mitochondrial enzyme catalyzes the transamidination of glycine by arginine, yielding guanidinoacetate and ornithine. This reaction represents the rate-limiting first step in the endogenous biosynthesis of creatine, a molecule critical for cellular energy buffering in tissues with high and fluctuating energy demands, such as skeletal muscle, cardiac muscle, and brain.

The GATM gene encodes a mature protein that is processed from a precursor form containing a mitochondrial targeting sequence. The recombinant protein supplied by Aladdin Scientific (SKU: rp369535) is intended to support in vitro biochemical and cell biology research applications.

Biological Significance and Research Applications

GATM is physiologically expressed at highest levels in the kidney and liver, where it supplies guanidinoacetate to the circulation for subsequent methylation to creatine in the liver by guanidinoacetate N-methyltransferase (GAMT). Deficiency of GATM in humans results in a rare autosomal recessive disorder characterized by intellectual disability, developmental delay, and myopathy due to creatine deficiency in the brain and muscle.

Recombinant Human GATM Protein is commonly used in the following research contexts:

• Enzyme kinetics and inhibitor screening studies targeting the creatine biosynthesis pathway
• Structural and biochemical characterization of amidinotransferase activity
• Investigation of GATM regulation in metabolic and rare disease models
• Development of assays for guanidinoacetate production
• Antibody validation and protein interaction studies

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human GATM Protein is essential to maintain enzymatic activity and structural integrity. As a general guideline applicable to most recombinant proteins of this class:

• Short-term storage (up to 1 month): Store at 4°C in the supplied buffer.
• Long-term storage: Aliquot and store at −20°C or −80°C to avoid repeated freeze-thaw cycles, which can lead to protein aggregation and activity loss.
• Reconstitution: If lyophilized, reconstitute in the recommended buffer (typically sterile PBS or the manufacturer-specified diluent) and allow the protein to equilibrate at room temperature before use.
• Carrier protein: Adding BSA (e.g., 0.1% final concentration) may improve stability during long-term storage at low protein concentrations.

Note: Always refer to the product datasheet for lot-specific storage conditions and recommended working concentrations.

Quality, Purity, and Regulatory Considerations

Recombinant proteins supplied for research use are typically characterized for purity by SDS-PAGE and may be further validated by Western blot or functional activity assays. For Recombinant Human GATM Protein (rp369535), researchers should review the certificate of analysis (CoA) accompanying each lot for:

• Purity assessment (e.g., ≥85% or ≥95% by SDS-PAGE, depending on the grade)
• Endotoxin levels (relevant for cell-based assays)
• Biological activity data, if provided
• Molecular weight confirmation

This product is supplied for research use only (RUO) and is not intended for diagnostic, therapeutic, or clinical applications. Procurement teams should confirm the grade and specification with the product datasheet prior to ordering.

Ordering and Technical Support

Recombinant Human GATM Protein is available from Aladdin Scientific under SKU rp369535. To place an order or request a quotation, researchers and procurement managers can contact the Aladdin Scientific sales team or visit the product page directly.

For technical inquiries — including questions about compatibility with specific assay formats, buffer optimization, or custom bulk quantities — please reach out to the Aladdin Scientific technical support team. Providing your experimental context will help our specialists recommend the most suitable product format and concentration.

When ordering, please reference:

• SKU: rp369535
• Supplier Reference: absea#PP-3876
• Product Name: Recombinant Human GATM Protein

Frequently asked questions

What is the function of the GATM protein?

GATM (glycine amidinotransferase) catalyzes the first step in creatine biosynthesis: the transfer of an amidino group from L-arginine to glycine, producing guanidinoacetate and ornithine. This reaction is the rate-limiting step in endogenous creatine production and occurs primarily in the kidney and liver.

What expression system is used to produce this recombinant GATM protein?

The specific expression system for rp369535 is not detailed in the provided source material. Common systems for recombinant human proteins include E. coli, baculovirus/insect cells, and HEK293 mammalian cells. Please consult the product datasheet or contact Aladdin Scientific technical support for confirmed expression system information.

What is the recommended storage condition for Recombinant Human GATM Protein?

For long-term storage, the protein should be aliquoted and stored at −20°C or −80°C to prevent activity loss from repeated freeze-thaw cycles. For short-term use within one month, storage at 4°C in the supplied buffer is generally acceptable. Always verify conditions on the lot-specific certificate of analysis.

Is this product suitable for use in cell-based assays?

Recombinant Human GATM Protein may be suitable for cell-based assays depending on the endotoxin level of the specific lot. Researchers should check the certificate of analysis for endotoxin specifications before using this protein in cell-based or in vivo experimental settings. Contact Aladdin Scientific technical support for guidance on your specific application.

What diseases or conditions are associated with GATM deficiency?

GATM deficiency causes a rare autosomal recessive inborn error of creatine metabolism. Affected individuals present with intellectual disability, developmental delay, expressive language impairment, and myopathy due to insufficient creatine synthesis in the brain and muscle. Supplementation with creatine monohydrate can partially improve symptoms in some patients.

How do I order Recombinant Human GATM Protein from Aladdin Scientific?

You can order Recombinant Human GATM Protein using SKU rp369535 through the Aladdin Scientific website or by contacting the sales team directly. For bulk orders, custom formats, or quotation requests, please reference the supplier code absea#PP-3876 and reach out to the procurement or technical support team.

References

1. Item, C.B. et al. (2001). AGAT (Glycine Amidinotransferase) Deficiency — a rare inborn error of creatine biosynthesis. General reference for GATM biology.
2. Wyss, M. & Kaddurah-Daouk, R. (2000). Creatine and creatinine metabolism. Physiological Reviews, 80(3), 1107–1213.
3. Aladdin Scientific Product Page: Recombinant Human GATM Protein, SKU rp369535, Supplier Ref absea#PP-3876.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.