Recombinant Human GBP7 Protein: Research Guide & Applications

Recombinant Human GBP7 Protein is a research-grade recombinant form of the human guanylate-binding protein 7, produced for use in studies of innate immune defense, interferon-gamma signaling, and GTPase-mediated antimicrobial responses.

Product Name

Recombinant Human GBP7 Protein

SKU

rp372908

Protein Family

Guanylate-Binding Protein (GBP) family, dynamin-like GTPase superfamily

Primary Research Area

Innate immunity, interferon-gamma response, antimicrobial defense

Expression System

Recombinant expression system (specific host to be confirmed with product datasheet)

Recommended Storage

Store at -80°C; avoid repeated freeze-thaw cycles (confirm with product datasheet)

Purity

>90% as determined by SDS-PAGE (confirm with product datasheet)

Biological Background of GBP7

Guanylate-binding protein 7 (GBP7) is a member of the GBP family, a group of large dynamin-like GTPases that are strongly induced by type I and type II interferons, particularly interferon-gamma (IFN-γ). The GBP family encompasses at least seven human members (GBP1–GBP7), all of which share a conserved N-terminal GTPase domain and a C-terminal helical domain responsible for protein dimerization and membrane association.

GBP7, like other family members, plays important roles in cell-autonomous innate immunity. GBPs are recruited to pathogen-containing vacuoles and disrupted membranes, contributing to the direct restriction of intracellular pathogens including bacteria, viruses, and parasites. GBP7 has been studied in the context of autophagy regulation, oxidative burst modulation, and interactions with components of the NADPH oxidase complex, distinguishing it functionally from other GBP family members.

Understanding GBP7's molecular functions requires high-quality recombinant protein for in vitro biochemical assays, structural studies, and antibody validation experiments.

Product Specifications and Quality

Recombinant Human GBP7 Protein (rp372908) from Aladdin Scientific is produced using a recombinant expression system to ensure batch-to-batch consistency and high purity suitable for demanding research applications. Researchers are advised to consult the official product datasheet for the most current and precise specifications, as parameters may be updated to reflect manufacturing improvements.

• Purity: Typically >90% as assessed by SDS-PAGE; endotoxin levels are controlled to minimize interference in cell-based assays (verify exact values on the product datasheet).
• Formulation: Supplied in a proprietary buffer optimized for protein stability; specific buffer composition and protein concentration are listed on the product datasheet.
• Molecular Weight: Consistent with the predicted molecular weight of human GBP7 based on its amino acid sequence; any tags or modifications are detailed in the datasheet.
• Activity: Functional activity data, where available, are provided in the product datasheet and may include GTPase activity assays.

All lots undergo quality control testing prior to release to ensure product integrity.

Handling, Storage, and Reconstitution Protocol

Proper handling of Recombinant Human GBP7 Protein is essential to preserve activity and ensure reproducible experimental results. Follow the guidelines below and always refer to the product-specific datasheet for authoritative instructions.

1. Receipt and Inspection: Upon receipt, immediately transfer the protein to the recommended storage temperature. Inspect the vial for integrity before use.
2. Storage: Store at -80°C for long-term storage. Working aliquots may be stored at -20°C for short-term use. Avoid storing at 4°C for extended periods, as protein degradation may occur.
3. Freeze-Thaw Cycles: Minimize freeze-thaw cycles. Prepare single-use aliquots upon first thaw to prevent repeated freeze-thaw, which can reduce protein activity and increase aggregation.
4. Reconstitution: If supplied in lyophilized form, reconstitute in the buffer recommended in the datasheet (typically sterile deionized water or a specific buffer). Allow the vial to equilibrate to room temperature before opening. Gently mix by pipetting; do not vortex vigorously.
5. Working Concentration: Centrifuge briefly before use if precipitate is observed. Determine optimal working concentration empirically for each assay type.
6. Sterile Technique: Use aseptic technique when handling protein intended for cell-based assays to prevent contamination.

Research Applications

Recombinant Human GBP7 Protein is suitable for a range of research applications in immunology, cell biology, and infectious disease research:

• In Vitro GTPase Activity Assays: Characterize the nucleotide hydrolysis activity of GBP7 and evaluate the effects of potential modulators or mutations on enzymatic function.
• Protein–Protein Interaction Studies: Use purified GBP7 in pull-down assays, co-immunoprecipitation, or surface plasmon resonance (SPR) experiments to identify binding partners relevant to innate immune signaling.
• Antibody Validation: Serve as a positive control antigen for validating GBP7-specific antibodies in Western blot, ELISA, or immunofluorescence assays.
• Structural Biology: Suitable as starting material for crystallography or cryo-electron microscopy studies aimed at resolving the three-dimensional structure of GBP7 alone or in complex with binding partners.
• Cell-Free Reconstitution: Reconstitute GBP7-dependent processes in cell-free systems to dissect molecular mechanisms of interferon-induced immunity.
• Drug Discovery Screening: Deploy in high-throughput assays to screen small-molecule libraries for compounds that modulate GBP7 activity, relevant to infectious disease or inflammatory disorder therapeutics.

Troubleshooting Common Issues

Researchers encountering difficulties with Recombinant Human GBP7 Protein may consult the following guidance. For unresolved issues, contact Aladdin Scientific technical support.

• Low or No GTPase Activity: Verify that the protein has not undergone excessive freeze-thaw cycles. Confirm that assay conditions (buffer composition, pH, divalent cation concentrations such as Mg²⁺) match those validated for GBP family proteins. Check that nucleotide substrate (GTP) is of appropriate quality and concentration.
• Protein Aggregation: Ensure protein is thawed gently on ice and centrifuged before use. If aggregation persists, consider adding a carrier protein (e.g., BSA) or optimizing buffer conditions. Do not vortex.
• Weak Signal in Western Blot: Confirm antibody compatibility with the recombinant construct; note whether a tag is present and select appropriate detection antibody. Ensure sufficient protein loading and appropriate transfer conditions for proteins of the expected molecular weight.
• High Background in ELISA: Optimize blocking conditions. Ensure that recombinant protein is properly diluted and that plate coating conditions are empirically determined.
• Unexpected Bands on SDS-PAGE: Minor bands may represent degradation products or dimeric forms. Consult the product datasheet for expected banding pattern and note whether reducing or non-reducing conditions are used.

Frequently asked questions

What is GBP7 and why is it important in research?

GBP7 (Guanylate-Binding Protein 7) is an interferon-induced dynamin-like GTPase involved in cell-autonomous innate immunity. It plays roles in restricting intracellular pathogens and has been linked to NADPH oxidase interactions and autophagy regulation, making it a valuable target for infectious disease and immunology research.

How should Recombinant Human GBP7 Protein be stored to maintain activity?

For long-term storage, keep the protein at -80°C. Avoid repeated freeze-thaw cycles by preparing single-use aliquots on first thaw. Short-term working stocks may be stored at -20°C. Always refer to the product datasheet for the exact storage recommendations for your specific lot.

What applications is Recombinant Human GBP7 Protein (rp372908) suitable for?

This recombinant protein is suitable for in vitro GTPase activity assays, protein–protein interaction studies (pull-down, SPR), antibody validation (Western blot, ELISA, immunofluorescence), structural biology studies, cell-free reconstitution experiments, and drug discovery screening campaigns targeting innate immune pathways.

What purity can I expect from this recombinant protein?

Recombinant Human GBP7 Protein from Aladdin Scientific (rp372908) is typically >90% pure as assessed by SDS-PAGE. Endotoxin levels are controlled for suitability in cell-based assays. Please consult the current product datasheet or contact technical support for lot-specific quality control data.

Can I use Recombinant Human GBP7 Protein as an antibody validation standard?

Yes. Recombinant Human GBP7 Protein is well-suited as a positive control antigen for validating GBP7-specific antibodies in assays such as Western blot, ELISA, and immunofluorescence. Ensure that the protein construct (including any affinity tags) is compatible with the antibody epitope being tested.

References

1. MacMicking JD. Interferon-inducible effector mechanisms in cell-autonomous immunity. Nature Reviews Immunology. 2012;12(5):367-382. doi:10.1038/nri3210
2. Praefcke GJ. Regulation of innate immune functions by guanylate-binding proteins. International Journal of Medical Microbiology. 2018;308(1):237-245. doi:10.1016/j.ijmm.2017.10.013
3. Vestal DJ, Jeyaratnam JA. The guanylate-binding proteins: emerging insights into the biochemical properties and functions of this family of large interferon-induced guanosine triphosphatase. Journal of Interferon & Cytokine Research. 2011;31(1):89-97. doi:10.1089/jir.2010.0079

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.