Recombinant Human GLIPR1L1 Protein: FAQs & Key Facts

Recombinant Human GLIPR1L1 protein is a member of the CRISP/PR-1/SCP superfamily involved in cell signaling and immune modulation, produced for use as a high-quality research reagent in studies of cancer biology, immune response, and protein–protein interaction.

Protein Name

Recombinant Human GLIPR1L1 (Glioma Pathogenesis-Related Protein 1-Like Protein 1)

SKU

rp367972

Protein Family

CRISP/PR-1/SCP superfamily (CAP superfamily)

Species Origin

Human (Homo sapiens)

Recommended Storage

Store at -80 °C; avoid repeated freeze-thaw cycles

Primary Research Areas

Cancer biology, immune modulation, cell signaling

What Is GLIPR1L1 and Why Is It Scientifically Relevant?

GLIPR1L1 (Glioma Pathogenesis-Related Protein 1-Like Protein 1) is a member of the CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related proteins) superfamily, also known as the CRISP/PR-1/SCP superfamily. Proteins in this family share a conserved CAP domain and are implicated in diverse biological processes including immune regulation, cell proliferation, and apoptosis.

GLIPR1L1 is expressed in a variety of human tissues and has been studied in the context of cancer biology, particularly regarding its potential roles in tumor suppression and immune evasion. As a secreted or membrane-associated protein, it may participate in extracellular signaling events relevant to both normal physiology and pathological states.

Research into GLIPR1L1 and related family members continues to shed light on molecular mechanisms underlying glioma and other malignancies, making recombinant GLIPR1L1 protein a valuable tool for biochemical and cell-based assays.

Product Specifications and Quality Attributes

Aladdin Scientific's Recombinant Human GLIPR1L1 protein (SKU: rp367972) is produced using established recombinant expression systems to ensure consistent batch-to-batch quality. Key attributes researchers should be aware of include:

• Origin: Human (Homo sapiens)
• Protein Family: CRISP/PR-1/SCP superfamily (CAP domain-containing)
• Format: Supplied as a lyophilized or liquid formulation (confirm with datasheet)
• Purity: Verified by SDS-PAGE and/or other analytical methods (refer to product datasheet for lot-specific values)
• Endotoxin Level: Tested and controlled to meet requirements for cell-based assays (refer to Certificate of Analysis)

Always consult the most current product datasheet and Certificate of Analysis (CoA) provided with your specific lot for exact specifications, as these may vary between production batches.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling are essential to maintain the activity and integrity of Recombinant Human GLIPR1L1 protein. Follow these best-practice guidelines:

• Long-term storage: Store at -80 °C in aliquots to prevent activity loss from repeated freeze-thaw cycles.
• Short-term storage: If the protein will be used within 1–2 weeks, it may be stored at 4 °C; however, -80 °C is recommended for extended stability.
• Avoid repeated freeze-thaw cycles: Prepare single-use aliquots upon first thaw to preserve biological activity.
• Reconstitution: For lyophilized product, reconstitute in the buffer recommended on the datasheet (typically sterile PBS or similar physiological buffer). Allow to equilibrate gently; do not vortex vigorously.
• Carrier proteins: Addition of a carrier protein such as 0.1% BSA may improve stability in some formulations; consult the product datasheet.

Always thaw on ice and centrifuge briefly before use to collect the full volume. If any visible precipitation or unusual color is observed, do not use the product and contact Aladdin Scientific technical support.

Research Applications of Recombinant Human GLIPR1L1 Protein

Recombinant Human GLIPR1L1 protein is suited for a range of in vitro and cell-based research applications, including but not limited to:

• Protein interaction studies: Used as bait or target protein in pull-down assays, co-immunoprecipitation, or surface plasmon resonance (SPR) experiments to identify binding partners.
• Functional assays: Addition to cell culture systems to investigate effects on cell proliferation, apoptosis, or immune cell activation.
• Antibody validation: Serves as a positive control antigen for validating anti-GLIPR1L1 antibodies by ELISA or Western blot.
• Structural studies: Provides purified material for circular dichroism (CD), size-exclusion chromatography (SEC), or other biophysical characterization methods.
• Cancer biology research: Investigates potential roles in tumor suppression, immune modulation, or signaling pathways associated with glioma and other cancers.

Researchers are encouraged to optimize assay conditions including protein concentration, buffer composition, and incubation time for their specific experimental system.

Ordering, Shipping, and Technical Support

Recombinant Human GLIPR1L1 protein is available from Aladdin Scientific under SKU rp367972. When placing an order, please note:

• Ordering: Orders can be placed through the Aladdin Scientific website or by contacting your regional sales representative. Provide the SKU and required quantity to ensure accurate fulfillment.
• Shipping: Recombinant proteins are shipped on dry ice to maintain cold-chain integrity. Confirm shipping conditions with your local Aladdin Scientific office, as regional regulations may apply.
• Lead time: Standard lead times apply; contact Aladdin Scientific for current stock availability and estimated delivery timelines.
• Technical support: For questions regarding product performance, reconstitution, or experimental design, contact Aladdin Scientific's technical support team with your lot number and CoA reference.
• Returns and quality issues: If you receive a product that does not meet the stated specifications, please contact customer service promptly with your order details and a description of the issue.

Frequently asked questions

What is the recommended storage condition for Recombinant Human GLIPR1L1 protein (rp367972)?

For long-term storage, keep Recombinant Human GLIPR1L1 protein at -80 °C in single-use aliquots to avoid activity loss from repeated freeze-thaw cycles. For short-term use within 1–2 weeks, storage at 4 °C is acceptable. Always consult the product datasheet and CoA for lot-specific recommendations.

What protein family does GLIPR1L1 belong to, and what is its biological significance?

GLIPR1L1 belongs to the CAP superfamily (also known as the CRISP/PR-1/SCP superfamily), characterized by a conserved CAP domain. Members of this family are involved in immune regulation, cell signaling, and apoptosis, and GLIPR1L1 has been studied for potential roles in cancer biology, including tumor suppression and immune modulation.

What applications is Recombinant Human GLIPR1L1 protein suitable for?

Recombinant Human GLIPR1L1 protein (rp367972) is suitable for protein interaction studies (e.g., pull-down, co-IP, SPR), functional cell-based assays, antibody validation by ELISA or Western blot, structural and biophysical characterization, and cancer biology research. Optimization of assay conditions is recommended for each specific application.

How should I reconstitute the lyophilized Recombinant Human GLIPR1L1 protein?

Reconstitute lyophilized GLIPR1L1 protein in the buffer specified on the product datasheet, typically sterile PBS or an equivalent physiological buffer. Mix gently by pipetting or swirling; avoid vigorous vortexing. Centrifuge briefly after reconstitution and aliquot for single use before storing at -80 °C.

How do I place an order for Recombinant Human GLIPR1L1 protein from Aladdin Scientific?

You can order Recombinant Human GLIPR1L1 protein (SKU: rp367972) through the Aladdin Scientific website or by contacting your regional sales representative. Please have the SKU number and required quantity ready. For stock availability and delivery timelines, contact Aladdin Scientific directly.

References

1. Gibbs GM, Roelants K, O'Bryan MK. The CAP superfamily: cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 proteins — roles in reproduction, cancer, and immune defense. Endocrine Reviews. 2008;29(7):865-897. doi:10.1210/er.2008-0032
2. Aladdin Scientific. Recombinant Human GLIPR1L1 protein, SKU rp367972. Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.