Recombinant Human GMFG Protein: FAQs & Key Facts

Recombinant Human GMFG Protein is a research-grade recombinant form of the human Glia Maturation Factor Gamma, used to study actin filament dynamics, cytoskeletal regulation, and related cell biological processes.

Product Name

Recombinant Human GMFG Protein

SKU

rp369734

Gene / Protein

GMFG (Glia Maturation Factor Gamma)

Species

Human (Homo sapiens)

Application

In vitro biochemical assays, cytoskeletal studies, binding assays

Format

Recombinant protein (lyophilized or solution; confirm with datasheet)

Supplier Reference

absea#PP-4085

What Is GMFG and Why Is It Important in Research?

GMFG, or Glia Maturation Factor Gamma, is a member of the actin depolymerizing factor (ADF)/cofilin superfamily of proteins. It plays a critical role in regulating actin filament dynamics by promoting the debranching and depolymerization of actin networks, particularly those nucleated by the Arp2/3 complex. GMFG is predominantly expressed in hematopoietic and immune cells, distinguishing it from the closely related GMFB isoform, which is more broadly expressed.

In research, GMFG is studied in the context of immune cell migration, inflammatory responses, and cytoskeletal remodeling. Dysregulation of GMFG has been associated with altered leukocyte trafficking and may have implications in inflammatory diseases. Recombinant Human GMFG Protein enables investigators to perform controlled biochemical experiments to dissect these mechanisms in vitro.

How Should Recombinant Human GMFG Protein Be Stored and Reconstituted?

Proper storage and reconstitution are essential for maintaining the activity and integrity of Recombinant Human GMFG Protein. As specific storage conditions are defined in the product datasheet (absea#PP-4085), always refer to the supplied documentation for authoritative guidance. General best practices for recombinant proteins of this type include:

• Storage: Store lyophilized protein at –20 °C or –80 °C, protected from moisture and repeated freeze–thaw cycles. Aliquot upon reconstitution to avoid degradation.
• Reconstitution: Reconstitute in sterile, deionized water or the buffer specified in the datasheet. Allow the vial to equilibrate to room temperature before opening. Gently pipette to mix; avoid vortexing.
• Working solution: Prepare fresh working dilutions in an appropriate buffer (e.g., PBS with carrier protein) and use promptly. Avoid prolonged exposure to room temperature.

Note: Always verify conditions against the official product datasheet, as formulation details may vary by lot.

What Are the Typical Applications of Recombinant Human GMFG Protein?

Recombinant Human GMFG Protein is suitable for a range of in vitro research applications, particularly those focused on cytoskeletal biology and immune cell function. Typical uses include:

• Actin dynamics assays: Investigating the debranching or depolymerization activity of GMFG on Arp2/3-nucleated actin networks using fluorescence-based or pyrene-actin polymerization assays.
• Protein–protein interaction studies: Examining binding interactions between GMFG and Arp2/3 complex subunits or other cytoskeletal regulators via pull-down, co-immunoprecipitation, or surface plasmon resonance (SPR).
• Functional rescue experiments: Supplementing GMFG-depleted cell lysates or systems to assess functional complementation.
• Antibody validation: Using recombinant GMFG protein as a positive control or antigen in Western blot, ELISA, or dot blot experiments.
• Structural and biophysical studies: Applying purified protein for X-ray crystallography, cryo-EM, or thermofluor stability assays.

Researchers should confirm suitability for each specific application by consulting the product datasheet and relevant literature.

Quality, Purity, and Validation of Recombinant Human GMFG Protein

Quality and purity are critical parameters when selecting a recombinant protein for research. While precise purity values and quality-control data for Recombinant Human GMFG Protein (SKU: rp369734) should be confirmed via the official product datasheet (absea#PP-4085), standard quality attributes for research-grade recombinant proteins typically include:

• Purity: Typically ≥90% as determined by SDS-PAGE and/or HPLC analysis. Exact values should be verified in the CoA (Certificate of Analysis).
• Endotoxin level: Tested and documented in the CoA; low endotoxin preparations are important for cell-based assays.
• Identity confirmation: Usually verified by mass spectrometry or N-terminal sequencing.
• Biological activity: May be assessed via actin debranching assays or other functional readouts where applicable.

Customers are encouraged to request the Certificate of Analysis (CoA) and Safety Data Sheet (SDS) for the specific lot number at the time of purchase. Contact Aladdin Scientific's technical support team for assistance.

Frequently asked questions

What is the biological function of GMFG?

GMFG (Glia Maturation Factor Gamma) is a member of the ADF/cofilin superfamily that regulates actin cytoskeleton dynamics. It promotes the debranching of Arp2/3-generated actin filament networks and facilitates actin depolymerization, playing a key role in immune cell migration and cytoskeletal remodeling.

What expression system is used to produce Recombinant Human GMFG Protein?

The specific expression system used for Recombinant Human GMFG Protein (SKU: rp369734) should be confirmed in the official product datasheet (absea#PP-4085). Common systems for proteins of this type include E. coli, baculovirus-insect cell, or mammalian cell expression. Please consult the datasheet or contact Aladdin Scientific technical support for lot-specific details.

How should I avoid loss of activity during storage and handling?

To preserve activity, store lyophilized Recombinant Human GMFG Protein at –20 °C or –80 °C and avoid repeated freeze–thaw cycles by preparing single-use aliquots after reconstitution. Use the buffer and storage conditions specified in the product datasheet, and always handle the protein on ice during experiments.

Is Recombinant Human GMFG Protein suitable for cell-based assays?

Recombinant Human GMFG Protein may be suitable for certain cell-based assays, particularly if a low-endotoxin preparation is used. Endotoxin levels are typically documented in the Certificate of Analysis. Researchers should confirm endotoxin specifications with Aladdin Scientific and validate performance in their specific cell model before use.

How do I order Recombinant Human GMFG Protein from Aladdin Scientific?

Recombinant Human GMFG Protein can be ordered directly from Aladdin Scientific using SKU rp369734. Visit the Aladdin Scientific website, search by SKU or product name, and add to your cart. For bulk orders, custom requirements, or technical inquiries, contact the Aladdin Scientific sales or technical support team.

References

1. Poukkula M, Kremneva E, Serlachius M, Lappalainen P. Actin-depolymerizing factor homology domain: a conserved fold performing diverse roles in cytoskeletal dynamics. Cytoskeleton (Hoboken). 2011;68(9):471-490. doi:10.1002/cm.20527
2. Ydenberg CA, Johnston A, Weinstein J, et al. Combinatorial genetic analysis of a network of actin disassembly-promoting factors. Cytoskeleton (Hob). 2015;72(7):349-361.
3. Aladdin Scientific product datasheet: Recombinant Human GMFG Protein, SKU rp369734, absea#PP-4085. Aladdin Scientific internal reference.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.