Recombinant Human GNL2 Protein: Research Guide & Applications

Recombinant Human GNL2 Protein is a research-grade recombinant form of the human GNL2 GTPase, a nucleolar protein essential for 60S ribosomal subunit biogenesis and cell proliferation, produced for use in biochemical, structural, and functional studies.

Product Name

Recombinant Human GNL2 Protein

SKU

rp368019

Protein Function

Nucleolar GTPase involved in 60S ribosomal subunit biogenesis and pre-rRNA processing

Host Organism

Recombinant expression system (verify with datasheet)

Primary Research Areas

Ribosome biogenesis, nucleolar biology, cell proliferation, cancer biology

Storage Recommendation

Store at -80°C; avoid repeated freeze-thaw cycles (verify with datasheet)

Biological Background of GNL2

GNL2 (G Protein Nucleolar 2), also known as NGP-1 (Nucleolar GTP-binding Protein 1), is a conserved member of the YTHDC/Obg family of GTP-binding proteins. It localizes predominantly to the nucleolus and is expressed in actively proliferating cells. GNL2 plays an essential role in the biogenesis of the large (60S) ribosomal subunit, participating in the processing of precursor ribosomal RNA (pre-rRNA) and the nuclear export of pre-60S particles.

Dysregulation of GNL2 expression has been associated with impaired cell proliferation and has attracted interest in the context of cancer biology, where ribosome biogenesis is frequently upregulated. Understanding GNL2 function at the molecular level requires access to high-quality recombinant protein for in vitro assays, interaction studies, and structural investigations.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human GNL2 Protein (SKU: rp368019) is produced using recombinant expression technology to ensure consistent batch-to-batch quality suitable for demanding research applications. Researchers should consult the product datasheet for confirmed details including:

• Expression system: Confirm host organism (e.g., E. coli, baculovirus, or mammalian cell system) on the product datasheet.
• Purity: Recombinant proteins from Aladdin Scientific are typically characterized by SDS-PAGE and other analytical methods. Refer to the Certificate of Analysis (CoA) for lot-specific purity data.
• Formulation: Buffer composition, glycerol content, and any carrier proteins are specified in the datasheet. Confirm formulation before use in sensitive assays.
• Molecular weight: Verify the expected molecular weight from the datasheet, accounting for any affinity tags used during purification.

Always review the CoA provided with each lot to confirm that the product meets stated specifications before use.

Handling, Reconstitution, and Storage Protocol

Proper handling of Recombinant Human GNL2 Protein is critical to maintaining activity and ensuring reproducible experimental results. Follow these general guidelines, and always defer to the specific instructions provided in the product datasheet:

1. Upon receipt: Inspect the package and product. If supplied lyophilized, briefly centrifuge the vial before opening. If supplied as a liquid, thaw gently on ice.
2. Reconstitution (lyophilized format): Reconstitute with the volume of sterile, ultrapure water or buffer as specified in the datasheet. Allow the solution to equilibrate for 10–15 minutes at room temperature, then mix gently by pipetting. Do not vortex.
3. Aliquoting: Prepare single-use aliquots immediately after reconstitution or first thaw to minimize freeze-thaw cycles, which can reduce protein activity and cause aggregation.
4. Storage: Store aliquots at -80°C for long-term storage. Working stocks may be held at -20°C for short periods if consistent with datasheet guidance. Avoid repeated freeze-thaw cycles.
5. Working concentrations: Determine optimal protein concentrations empirically for each assay type. Typical ranges for recombinant GTPases vary by application; consult literature and validate in your specific experimental system.

Use of protein stabilizers such as BSA (if compatible with your assay) and the addition of reducing agents (e.g., DTT) may help maintain protein integrity during extended experiments. Verify compatibility with the datasheet formulation.

Research Applications

Recombinant Human GNL2 Protein is suitable for a range of in vitro research applications. Representative uses include:

• GTPase activity assays: GNL2 possesses GTPase activity that can be characterized in vitro using colorimetric or radioactive GTP hydrolysis assays, enabling studies of enzymatic kinetics and inhibitor screening.
• Protein–protein interaction studies: GNL2 interacts with multiple ribosome assembly factors and pre-60S components. Pull-down assays, co-immunoprecipitation, and surface plasmon resonance (SPR) experiments can be performed using recombinant GNL2 as bait or prey.
• Structural biology: High-purity recombinant GNL2 protein can be used in X-ray crystallography, cryo-EM, or NMR studies aimed at elucidating its three-dimensional structure and GTP-binding mechanisms.
• Antibody validation: Recombinant GNL2 serves as a positive control antigen for validating anti-GNL2 antibodies by Western blot, ELISA, or dot blot.
• Cell-free reconstitution: Recombinant GNL2 may be incorporated into cell-free ribosome assembly systems to study its specific role in pre-60S maturation steps.

Researchers should validate the protein in their specific assay format prior to use in critical experiments. Optimization of buffer conditions, pH, temperature, and cofactors (e.g., Mg²⁺ for GTPase activity) is recommended.

Quality Assurance and Regulatory Considerations

Aladdin Scientific's Recombinant Human GNL2 Protein is intended exclusively for research use and is not approved for diagnostic or therapeutic applications in humans or animals. The following quality and compliance considerations apply:

• Research Use Only (RUO): This product is labeled for research use only and must not be used in clinical procedures.
• Certificate of Analysis: Each lot is accompanied by a CoA specifying purity, concentration, and quality control test results. Retain the CoA for laboratory records.
• Biosafety: Handle all recombinant proteins in accordance with your institution's biosafety guidelines. Although this protein is of human origin, standard laboratory precautions (gloves, eye protection, appropriate containment) are required.
• Lot-to-lot consistency: While Aladdin Scientific maintains stringent manufacturing standards, researchers performing longitudinal studies should reserve sufficient material from a single lot where possible, or validate new lots before switching.

For technical support, batch-specific documentation, or custom production inquiries, contact Aladdin Scientific's technical support team.

Frequently asked questions

What is GNL2 and why is it important in ribosome biogenesis?

GNL2 (G Protein Nucleolar 2) is a conserved nucleolar GTPase required for the biogenesis of the 60S ribosomal subunit. It participates in pre-rRNA processing and the nuclear export of pre-60S particles. Its essential role in ribosome production makes it important for cell proliferation and a potential area of interest in cancer biology research.

How should I store Recombinant Human GNL2 Protein to maintain its activity?

For long-term storage, keep aliquots at -80°C. Prepare single-use aliquots to avoid repeated freeze-thaw cycles, which can degrade protein activity and promote aggregation. For short-term use, -20°C may be acceptable if indicated by the product datasheet. Always consult the lot-specific datasheet and CoA for confirmed storage conditions.

What applications is Recombinant Human GNL2 Protein validated for?

Recombinant Human GNL2 Protein is suitable for in vitro research applications including GTPase activity assays, protein–protein interaction studies (pull-down, SPR), antibody validation (Western blot, ELISA), and structural biology studies. Researchers should validate the protein in their specific assay format and consult the product datasheet for confirmed validated applications for each lot.

Can Recombinant Human GNL2 Protein be used for clinical or diagnostic purposes?

No. Recombinant Human GNL2 Protein (rp368019) is labeled for Research Use Only (RUO) and is not intended or approved for use in diagnostic, therapeutic, or clinical procedures in humans or animals.

How do I confirm the purity and quality of a specific lot of Recombinant Human GNL2 Protein?

Each lot of Recombinant Human GNL2 Protein is supplied with a Certificate of Analysis (CoA) that documents purity, concentration, and quality control data. Review the CoA upon receipt and contact Aladdin Scientific's technical support if you require additional batch-specific documentation or have quality concerns.

References

1. Leipe DD, Wolf YI, Koonin EV, Aravind L. Classification and evolution of P-loop GTPases and related ATPases. J Mol Biol. 2002.
2. Kressler D, Hurt E, Bassler J. Driving ribosome assembly. Biochim Biophys Acta. 2010.
3. Sydorskyy Y, Dilworth DJ, Yi EC, et al. Intersection of the Kap123p-mediated nuclear import and ribosome export pathways. Mol Cell Biol. 2003.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.