Recombinant Human MEAF6 Protein: FAQs & Key Facts

Recombinant Human MEAF6 Protein is a research-grade recombinant protein corresponding to a conserved subunit of histone acetyltransferase complexes, offered by Aladdin Scientific to support studies in chromatin biology, epigenetic regulation, and protein–protein interaction research.

Product Name

Recombinant Human MEAF6 Protein

SKU

rp369506

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant protein

Primary Function

Subunit of histone acetyltransferase (HAT) complexes involved in chromatin remodeling and transcriptional regulation

Supplier Reference

absea#PP-2781

What Is MEAF6 and Why Is It Scientifically Important?

MEAF6 (MYST/Esa1-associated factor 6) is a conserved non-catalytic subunit found within multiprotein histone acetyltransferase (HAT) complexes, most notably the NuA4/Tip60 HAT complex in humans. These complexes play critical roles in acetylating histone H4 and H2A, thereby regulating chromatin accessibility and gene transcription. MEAF6 orthologs are present from yeast to humans, underscoring its fundamental role in epigenetic regulation. Research into MEAF6 contributes to understanding transcriptional activation, DNA damage response, and cell cycle progression.

Product Specifications and Quality Standards

Aladdin Scientific's Recombinant Human MEAF6 Protein (SKU: rp369506) is produced to research-grade standards. Specific details regarding expression system, tag configuration, molecular weight, purity grade, and endotoxin levels are defined in the product datasheet associated with supplier reference absea#PP-2781. Researchers are advised to consult the accompanying Certificate of Analysis (CoA) for lot-specific quality data, including purity confirmation by SDS-PAGE or HPLC and biological activity validation where applicable.

For the most accurate and up-to-date specifications, please refer to the official product page on the Aladdin Scientific website or contact technical support directly.

Recommended Applications for Recombinant Human MEAF6 Protein

Recombinant Human MEAF6 Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–protein interaction studies: Investigating MEAF6 binding partners within HAT complexes or novel interactors.
• Biochemical assays: Use as a positive control or component in reconstituted complex assays.
• Antibody validation: Serving as an antigen standard to validate MEAF6-specific antibodies.
• Structural biology: As a component for crystallography or cryo-EM studies of HAT complex subunits.
• Functional studies: Exploring the role of MEAF6 in transcriptional regulation and chromatin remodeling pathways.

Specific validated applications and recommended working concentrations are listed in the product datasheet. Researchers should perform application-specific optimization.

Storage, Handling, and Stability Recommendations

Proper storage and handling of recombinant proteins are critical to maintaining activity and integrity. General best-practice recommendations for recombinant proteins of this class include:

• Store the protein at –80 °C for long-term preservation upon receipt.
• Avoid repeated freeze–thaw cycles; aliquot into single-use volumes before freezing.
• Briefly centrifuge the vial before opening to consolidate the contents.
• Reconstitute lyophilized protein in the buffer specified in the datasheet; allow to dissolve gently without vortexing.
• Working aliquots may be stored at 4 °C for short-term use (typically up to one week), but stability should be confirmed per the product datasheet.

Always refer to the specific storage conditions stated on the product label and CoA, as formulation details may vary by lot.

Ordering, Availability, and Technical Support

Recombinant Human MEAF6 Protein is available from Aladdin Scientific under SKU rp369506. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative. For bulk quantities or custom formulations, please reach out to the sales team directly.

Technical support, including guidance on experimental design, troubleshooting, and application-specific recommendations, is available through Aladdin Scientific's scientific support team. Certificate of Analysis (CoA) documents for specific lots can be downloaded from the product page or requested via customer service.

Returns and replacements are handled in accordance with Aladdin Scientific's standard return and refund policy. Please contact customer service with your order number and a description of any quality concern.

Frequently asked questions

What is Recombinant Human MEAF6 Protein and what is its biological role?

Recombinant Human MEAF6 Protein is a laboratory-produced form of the human MEAF6 protein, a conserved subunit of histone acetyltransferase (HAT) complexes such as the NuA4/Tip60 complex. It participates in chromatin remodeling by contributing to the acetylation of histones H4 and H2A, which modulates gene transcription, DNA repair, and cell cycle regulation.

What applications is Recombinant Human MEAF6 Protein validated for?

This protein is intended for research use and is suitable for applications including protein–protein interaction studies, biochemical reconstitution assays, antibody validation, and structural biology experiments. Specific validated applications are detailed in the product datasheet; researchers should consult the datasheet and perform optimization for their specific experimental conditions.

How should Recombinant Human MEAF6 Protein be stored to maintain its integrity?

For long-term storage, keep the protein at –80 °C and avoid repeated freeze–thaw cycles by preparing single-use aliquots prior to freezing. Short-term working stocks may be held at 4 °C for up to one week. Always follow the specific storage instructions provided on the product label and Certificate of Analysis.

What quality controls are applied to this recombinant protein?

Recombinant Human MEAF6 Protein is produced to research-grade standards; quality parameters such as purity, molecular weight confirmation, and endotoxin levels are assessed and reported in the lot-specific Certificate of Analysis (CoA). Please download the CoA from the Aladdin Scientific product page or contact customer service to obtain it.

How can I order Recombinant Human MEAF6 Protein and what is the SKU?

Recombinant Human MEAF6 Protein can be ordered directly through the Aladdin Scientific website or by contacting your regional sales representative. The product SKU is rp369506. For bulk inquiries or custom formulations, please contact the sales team for a tailored quote.

References

1. Doyon Y, Côté J. The highly conserved and multifunctional NuA4 HAT complex. Curr Opin Genet Dev. 2004;14(2):147-154. doi:10.1016/j.gde.2004.02.009
2. Sapountzi V, Côté J. MYST-family histone acetyltransferases: beyond chromatin. Cell Mol Life Sci. 2011;68(7):1147-1156. doi:10.1007/s00018-010-0599-9
3. Aladdin Scientific. Recombinant Human MEAF6 Protein, SKU rp369506 [Product datasheet]. Aladdin Scientific; accessed 2024.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.