What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
Frequently Asked Questions: Products, Experiments and Troubleshooting
What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
·Published ·Updated
What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
The precipitation in the denatured protein buffer may be related to the ion concentration in the protein sample. If the precipitation occurs during the temperature change, it can be dissolved by incubation in a water bath at 37℃ or below. If there is still a small amount of precipitate that cannot be dissolved the supernatant can be centrifuged and used.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
Cite this article
Aladdin Scientific. "What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/us_pt/faqs/the-protein-added-to-the-buffer-cooks-without-precipitation-en.html
Was this article helpful?YesNo47 out 88 found this helpful
Usamos cookies para garantir o funcionamento adequado do site e, quando permitido, para melhorar a sua experiência. Você pode gerenciar suas preferências a qualquer momento nas Configurações. Saiba mais em nossa Política de Cookies.
Deseja que lhe enviemos uma mensagem quando tivermos descontos disponíveis?
Lembrar-me mais tarde
Obrigado! Por favor, verifique a sua caixa de entrada de e-mail para confirmar.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
