MAb de ratinho GNMT - Anticorpo primário, specific to GNMT, Mouse IgG2a

Cat. No.: Ab105966

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry, WB

Host species

Rato

Reactive species

Humano, Rato

Isotype

Mouse IgG2a

Clone Number

A41S18

Conjugation

Unconjugated

Visão geral

O Anticorpo Mouse anti GNMT Humano, Monoclonal (A41S18), pode ser utilizado para WB, Flow e assim por diante.

Specifications

Product Name

MAb de ratinho GNMT - Anticorpo primário, specific to GNMT, Mouse IgG2a

Sinónimos

Anticorpo EC 2.1.1.20 | Anticorpo Li 182mP da proteína de ligação do esperma secretório do epidídimo | Anticorpo Glycine N methyltransferase | Anticorpo Glycine N-methyltransferase | Anticorpo Gnmt | Anticorpo GNMT_HUMAN | Anticorpo HEL S 182mP | Anticorp

Especificações e pureza

ExactAb™, validado, sem suporte, 0,5 mg/mL

Mecanismos bioquímicos e fisiológicos

Catalisa a metilação da glicina utilizando S-adenosilmetionina (AdoMet) para formar N-metilglicina (sarcosina) com a produção concomitante de S-adenosilhomocisteína (AdoHcy). Possível papel crucial na regulação da concentração tecidular de AdoMet a

Espécie hospedeira

Rato

Especificidade

GNMT

Imunogénio

Recombinant protein of human GNMT.

Controlo positivo

WB: Mouse liver lysates. Flow: U-2 OS cells.

Conjugação

Unconjugated

Grau

Carrier Free, ExactAb™, Validated

Isótipo

Mouse IgG2a

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein G purified

Concentração

0.5 mg/mL

Armazenamento e envio

Forma

Líquido

Condições de armazenamento de armazenamento

Conservar a -20°C. Evitar a congelação e descongelação repetidas

Enviado em

Arca de gelo + almofadas de gelo

Estabilidade e armazenamento

Armazenar a 4 ℃ curto prazo (1-2 semanas). Armazenar a -20 ℃ a longo prazo (12 meses). Após a receção, recomenda-se a alíquota. Evite o ciclo de congelamento / descongelamento.

Imagens

Western Blot: GNMT Mouse mAb (Ab105966)

GNMT Mouse mAb (Ab105966) - Western Blot
All lanes: GNMT Mouse mAb (Ab105966) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 31 kDa
Exposure time: 20.8 s

Flow Cytometry: GNMT Mouse mAb (Ab105966)

GNMT Mouse mAb (Ab105966) - Flow Cytometry
Flow cytometric analysis of U-2 OS cells using GNMT Mouse mAb (Ab105966). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37℃ for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37℃ for 40 min. Isotype control antibody (blue line) was used under the same condition.

Aplicação

• Aplicação: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/10-1/50;

Alvos associados (humanos)

GNMT Tchem Glycine N-methyltransferase (0 Activities)

Discover Target: GNMT

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GNMT Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.