Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.

🌡

Armazenamento e envio

Store at -20°C,Avoid repeated freezing and thawing Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.

📋

Documentos de qualidade

SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.

📚

Comprovação documental

Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.

Visão geral

Mouse anti Human HDAC1 Antibody, Monoclonal (E22), could be used for WB, IHC, IF, ICC and so on.

Application:

WB: 0.2-2μg/mL

IHC: 5-20μg/mL

ICC/IF: 5-20μg/mL

Protein Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

Post-translational

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.

Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.

Ubiquitinated by CHFR, leading to its degradation by the proteasome.

Especificações

Product Name

HDAC1 Mouse mAb - Anticorpo primário, specific to HDAC1, Mouse IgG1

Sinónimos

Especificações e pureza

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Espécie hospedeira

Rato

Especificidade

HDAC1

Imunogénio

Recombinant HDAC1 expressed in E.coli (AA 1-482) .

Controlo positivo

WB: A549, HeLa, HepG2, Jurkat, THP-1, HEK293 and NIH/3T3 cell lysates; IHC: Human Placenta Tissue, Human Lung cancer Tissue, Rat Colon Tissue, Rat Testis Tissue, Mouse Pancreas Tissue, Mouse Cerebellum Tissue; IF: HeLa.

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A+G Affinity Chromatography

Concentração

See COA

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: HDAC1 Mouse mAb (Ab107009)

HDAC1 Mouse mAb (Ab107009) - Western Blot
All lanes: HDAC1 Mouse mAb (Ab107009) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 63 kDa
Exposure time: 1.2 s