GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Visão geral
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Human B7-1/CD80 concentration in samples. The Human B7-1/CD80 capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Human B7-1/CD80 present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Human B7-1/CD80 is added. This antibody binds to the captured Human B7-1/CD80, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Human B7-1/CD80 is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofHuman B7-1/CD80 is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofHuman B7-1/CD80, and the concentration of Human B7-1/CD80 in unknown samples is interpolated from this curve based on their OD values.
Specifications
Especificações e pureza
BioReagent
Grau
BioReagent
Aplicação
ELISA
Tipo de amostra
fluidos corporais, homogeneizados de tecido, Plasma, sobrenadantes de cultura celular, Soro
Gama de deteção
62.5 pg/mL~4,000 pg/mL
Método de deteção
Sandwich ELISA
Espécie detectada Objeto
Human
Especificidade
Detecting Human B7-1/CD80 in samples, with no significant cross-reactivity to other homologs.
Detection instrument
Microplate reader
Detection wavelength
450nm
Armazenamento e envio
Condições de armazenamento de armazenamento
Store at 2-8°C
Enviado em
Wet ice
Estabilidade e armazenamento
Store at 2-8℃ for 6 months.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
