HyT36 - Moligand™, 10 mM in DMSO , CAS No.1323151-45-6

CAS: 1323151-45-6 Cat. No.: H1496912 PubChem CID: 52953469

GRADE & PURITY Moligand™, 10 mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Visão geral

HyT36 is a low molecular weight hydrophobic tag that promotes the degradation of fusion protein and pseudokinase Her3.

Specifications

Especificações e pureza

Moligand™, 10 mM in DMSO

Condições de armazenamento de armazenamento

Store at -80°C

Enviado em

Dry ice packs + Cold packs

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Grau

Moligand™

Tipo de ação

DEGRADER

Nomes e identificadores

SMILES isoméricas

C[C@H](CCC12CC3CC(C1)CC(C3)C2)C(=O)NCCOCCOCCCCCCCl

PubChem CID

52953469

Application Protocols

No validated in-house application protocols or recommended dilutions are provided for this item.

General guidance for small-molecule tool compounds (non-item-specific):

• Prepare a concentrated stock in dry DMSO (e.g., 10–50 mM as solubility allows).
• For cell assays, dilute into pre-warmed media with vigorous mixing to the desired final concentration, keeping DMSO at a low percentage (commonly ≤0.1–0.5% v/v). Include matched vehicle controls.
• Typical exposure windows range from 1–24 h for acute responses and up to 48–72 h for degradation time-course studies; optimize empirically and verify cell health.
• Assess target protein levels with orthogonal readouts (e.g., immunoblot, reporter signal) and include pathway controls as applicable (e.g., proteasome inhibitors) to interpret mechanism.

Please consult peer-reviewed literature for HyT-class experimental examples and rely on your institutional SOPs.

Biological Roles

Context from literature (general, non-item-specific):

• HyT-class molecules are described as hydrophobic tags that promote the selective degradation of target proteins. By appending or recruiting a bulky hydrophobic motif to a protein (commonly via a tagging system), these compounds mimic exposed hydrophobic surfaces associated with misfolded proteins, thereby engaging cellular protein quality-control machinery and the ubiquitin–proteasome system or related pathways.
• In cellular studies, HyT tools have been used to enable chemical knockdown of proteins of interest, providing temporal control complementary to genetic approaches. This strategy can facilitate studies of protein function, pathway mapping, and validation of targets in living cells.

Important caveats:

• The precise molecular target recognition mode for HyT36, any required tagging system, and potency/selectivity metrics are not provided in the Product Data and must be verified from primary literature and the CoA/Spec Sheet.
• Cellular responses can depend strongly on cell type, expression level of the tagged protein, proteostasis capacity, compound exposure time, and concentration. Pilot titrations and orthogonal readouts (e.g., immunoblot for the protein of interest and proteasome inhibition controls) are recommended.

No medical or clinical claims are made. This product is intended strictly for research use.

Buffer Applications

This compound is not a buffering agent and does not define a physiological pH range. Accordingly, there are no standard buffer recipes associated with HyT36.

Practical guidance for assay buffers (general):

• Use your system-appropriate biological buffer (e.g., PBS, HBSS, HEPES-buffered media) and introduce HyT36 from a concentrated stock in DMSO or another suitable co-solvent to keep the final organic solvent content low (commonly ≤0.1–0.5% v/v).
• Include vehicle-only controls at the same solvent percentage.
• If precipitation is observed on dilution, increase mixing, add the stock more slowly, or consider a small amount of compatible co-solvent while monitoring biological tolerance.

Green Alternatives

Green solvent/reagent substitution frameworks are generally aimed at reaction media and process chemicals. HyT36 is a discrete bioactive small molecule supplied for research use; as such, there is no direct “green alternative” to the compound itself without altering experimental objectives.

Practical sustainability considerations (general):

• Use the minimum effective amount of DMSO or other organic co-solvent in assay buffers to reduce solvent burden.
• Prepare concentrated stocks to minimize repeated thaw/freeze and waste; aliquot to right-size volumes.
• When possible, select assay solvents with favorable environmental profiles (e.g., ethanol over DMF) consistent with your biological system’s tolerance.
• Dispose of unused solutions and contaminated consumables as hazardous waste according to institutional and local regulations.

Note: Any substitution of the active probe (e.g., changing to a different HyT-class molecule or to an orthogonal degrader chemistry) will change experimental outcomes and should be guided by your scientific objective rather than green metrics alone.

Pharmaceutical Uses

No pharmacopeial or clinical/formulation uses are specified for this item. HyT36 is offered strictly for research applications within discovery and chemical biology.

Context (general):

• Tool compounds of this class may be used in early-stage discovery to interrogate target validity, mechanism of action, and degrader strategies. Such use is preclinical and research-only.
• This product is not manufactured to GMP standards and is not intended as an active pharmaceutical ingredient (API) or excipient.

For any development beyond bench research, consult appropriate regulatory guidance and source materials made to the required quality standards.

Physical Properties

Item-specific physicochemical constants are not provided in the Product Data.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point (MP): Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point (BP): Not applicable/unknown for a complex bioactive small molecule; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not applicable to solids; otherwise not specified for this item.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• LogP / cLogP: Not specified for this item; refer to CoA/Spec Sheet.
• pKa: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general context (non-item-specific):

• HyT-class ligands are commonly reconstituted in anhydrous DMSO for stock solutions due to their hydrophobic character; final working solutions are often prepared by diluting into aqueous buffer containing serum or a co-solvent (e.g., 0.1–1% DMSO). Actual solubility limits and stability for HyT36 must be verified experimentally and from the item’s documentation.

Practical notes:

• If the compound is provided as a solid, allow equilibration to room temperature in a desiccated environment before opening to minimize moisture condensation.
• Filter sterilization of working solutions (e.g., 0.22 µm) can improve solution clarity for cell-based assays, subject to compound stability.

Quality and Grades

• Grade/Purity: Moligand™ (as provided in Product Data).

What this means (context and expectations):

• Moligand™ denotes a research-use small-molecule ligand classification within a compound library context. It indicates a tool-compound grade intended for discovery research, assay development, and mechanistic studies. It is not a pharmacopeial or GMP grade and is not intended for clinical or diagnostic use.
• Typical quality controls for such items include identity verification (e.g., NMR/LC–MS), and purity by chromatographic methods. However, item-specific acceptance criteria and lot-specific results are not listed here.

Item-specific details:

• Stabilizers, salt form, residual solvents, water content, metal limits, UV cutoff: Not specified for this item; refer to CoA/Spec Sheet.
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.

Recommendations to end users:

• Request or download the lot-specific CoA for precise purity, analytical method, and identity confirmation data.
• For quantitative applications (e.g., dose–response studies), verify concentration by an orthogonal method if feasible (e.g., quantitative NMR or calibrated LC–UV) and assess solution stability under your assay conditions.
• Record and retain lot numbers to ensure reproducibility across studies.

Reaction and Applications

This product is a research small-molecule tool and is not typically employed as a stoichiometric reagent, catalyst, solvent, or building block in synthetic organic transformations.

• Not commonly used in classical reaction families (e.g., cross-coupling, oxidations, reductions) as a reagent.
• If your use case involves conjugation or derivatization, consult the CoA/Spec Sheet to confirm the presence of any functional handles and assess compatibility with planned chemistries.

For laboratory users, the more relevant aspects for HyT36 are covered under:

• Biological Roles (chemical biology context and conceptual mechanism),
• Storage & Reconstitution (practical handling), and
• Solvent Selection (preparing solutions for assays).

Reaction Conditions

This product is not intended as a reagent or catalyst, and no standard synthetic reaction conditions are associated with it.

General lab handling conditions for preparing assay solutions (non-item-specific):

• Dissolve in a suitable solvent (DMSO commonly) at room temperature with gentle agitation or brief sonication.
• Filter if needed (0.2–0.22 µm PTFE or PVDF), provided the compound is stable under those conditions.
• Avoid prolonged exposure to elevated temperatures or strong light unless stability is documented.

For any chemical transformations involving this molecule, consult primary structural data (CoA/Spec Sheet) and perform small-scale feasibility tests before committing valuable material.

Safety and Handling

GHS details for this specific item were not provided.

• Signal word / GHS classification / Pictograms: Not specified for this item; refer to SDS.
• Hazard statements (H-phrases): Not specified for this item; refer to SDS.
• Research use note: For research use only.

General laboratory precautions (good practice; defer to SDS):

• Handle in a chemical fume hood. Avoid inhalation of dust/aerosols and contact with skin/eyes.
• Wear suitable PPE: lab coat, safety glasses, and chemical-resistant gloves (nitrile recommended). Change gloves regularly and immediately upon contamination.
• Avoid sources of ignition and incompatible reagents until compatibility is known (strong oxidizers and strong acids/bases are common incompatibilities for organic small molecules).
• Use only clean, dry tools for weighing; minimize exposure of the container to ambient air to reduce moisture uptake or degradation.

First-aid overview (general):

• Skin/eye contact: Rinse with copious water for at least 15 minutes; remove contaminated clothing; seek medical attention.
• Inhalation: Move to fresh air; seek medical attention if symptoms persist.
• Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.

Waste and spill response:

• Absorb small spills with inert material (e.g., vermiculite). Collect as hazardous chemical waste according to local regulations.

Always consult the official SDS supplied with the lot for authoritative hazard, toxicology, and response information.

Solvent Selection

Rational solvent selection optimizes stock preparation and assay performance for hydrophobic library compounds.

General guidance (not item-specific):

• Primary solvent for stock: Anhydrous DMSO is typically preferred for hydrophobic small molecules due to broad solvency, miscibility with water at low percentages, and cell-compatibility at ≤0.1–0.5% v/v in most assays.
• Alternatives: DMF (good solvency but less common for live-cell work), ethanol or isopropanol (miscible with water; monitor for precipitation upon dilution), or acetonitrile (HPLC-friendly; watch for volatility and cell tolerance).
• Dilution strategy: Prepare a concentrated DMSO stock (e.g., 10–50 mM, as solubility allows), then dilute into buffer or media while vortexing. Add compound last to a vigorously mixed medium to minimize local supersaturation.

Solvent properties (literature, for planning):

• DMSO: polar aprotic; fully miscible with water; bp ~189 °C; reduces precipitation upon aqueous dilution compared with alcohols.
• Ethanol: protic; miscible with water; may affect membrane properties at >0.5–1% v/v.

Practical tips:

• Warm solutions gently (ambient to 37 °C) and sonicate briefly to assist dissolution; avoid prolonged heating.
• Use 0.2–0.22 µm filtration for clear working solutions if chemically compatible.
• For adsorption-sensitive assays, pre-rinse plasticware with dilute DMSO or use low-bind consumables.

Note: Actual solubility limits and stability of HyT36 in any solvent are not specified for this item; consult the CoA and verify empirically.

Storage and Reconstitution

Item-specific storage and shipping:

• Storage Conditions: Store at −80 °C (as provided in Product Data).
• Shipped In: Dry ice packs + Cold packs (as provided in Product Data).

General reconstitution and handling guidance (non-item-specific; verify experimentally):

• Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.
• Dissolve the compound in anhydrous DMSO to prepare a concentrated stock (e.g., 10–50 mM, as solubility permits). Mix gently or sonicate briefly to assist dissolution. Actual solubility for HyT36 is not specified for this item.
• Aliquot the stock into single-use volumes in low-bind tubes to avoid repeated freeze–thaw cycles.
• Store aliquots at −80 °C, protected from light and moisture. Under these conditions, many small-molecule stocks are stable for months; however, stability of HyT36 is not specified for this item and should be confirmed empirically.
• On the day of use, thaw on ice or at room temperature, mix thoroughly, and inspect for precipitation or discoloration.
• For aqueous working solutions, add the DMSO stock slowly to vigorously mixed buffer or media to minimize precipitation. Use promptly; avoid prolonged storage of diluted solutions.

Always refer to the lot-specific CoA/SDS for definitive storage, stability, and handling information.

Structure and Identity

Concise identity for a research-use small-molecule tool compound.

• Product Name: HyT36 (SKU: H1496912)
• CAS: 1323151-45-6
• PubChem CID: 52953469
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural notes (literature, general):

• HyT-series compounds are described in the chemical biology literature as hydrophobic-tag (HyT) ligands used to promote degradation of tagged proteins via protein quality-control pathways. Specific substructures and stereochemistry for HyT36 should be confirmed from the primary reference or the item’s CoA.

2D-structure description (general):

• HyT tools typically feature a recognition/anchoring element linked to a bulky hydrophobic moiety through a flexible spacer; this overall architecture is designed to mimic exposed hydrophobic surface, flagging the bound protein for degradation. Exact ring systems, functional groups, and stereochemical centers for HyT36 are not specified here and must be verified from the CoA/Spec Sheet.

Synthetic Utility

HyT36 is supplied as a functional small-molecule tool rather than a synthetic intermediate.

• There are no item-specific data indicating protected functional handles, leaving-group motifs, or orthogonal reactivity intended for downstream synthesis.
• If users plan to derivatize or immobilize the compound (e.g., for pull-downs or imaging), they should consult the CoA/Spec Sheet to confirm structural features and assess feasibility.

Given its intended role as a probe compound, please refer instead to Biological Roles and Storage & Reconstitution for the most relevant information.

Target Specificity

Target, binding partner, or tag specificity has not been provided for this item in the Product Data.

• Any association of HyT36 with a particular tagging system or protein target should be confirmed from the primary literature and the lot-specific documentation.
• No antibody/biologic attributes (epitope, isotype, species reactivity) apply to this small-molecule product.

Perguntas frequentes

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1323151-45-6.

What is Moligand??

Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.